Investigation on Antioxidant Activity and Different Metabolites of Mulberry ( Morus spp.) Leaves Depending on the Harvest Months by UPLC-Q-TOF-MS with Multivariate Tools

The changes in active components in mulberry leaves harvested in different months and their antioxidant activities were investigated. Ultra-high-performance liquid chromatography-tandem quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) with multivariate statistical tools was used to invest...

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Published in:Molecules (Basel, Switzerland) Switzerland), 2023-02, Vol.28 (4), p.1947
Main Authors: Guo, Zili, Lai, Jiangxuan, Wu, Yiwen, Fang, Sheng, Liang, Xianrui
Format: Article
Language:English
Subjects:
Amino acids
antioxidant
Antioxidants
Aqueous solutions
Bioavailability
Biological activity
chemical constituent
Discriminant analysis
Ethanol
Fatty acids
Flavonoids
free radical scavenging activity
High performance liquid chromatography
Isoflavones
Leaves
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Metabolites
Morus
mulberry leaf
Multivariate analysis
Organic acids
Oxidation
Phenolic acids
Phenols
Quadrupoles
Scavenging
UPLC–Q-TOF-MS
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Summary:The changes in active components in mulberry leaves harvested in different months and their antioxidant activities were investigated. Ultra-high-performance liquid chromatography-tandem quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) with multivariate statistical tools was used to investigate the chemical constituents in the extracts of mulberry leaves. The results indicated that mulberry leaves were rich in phenolic acids, flavonoids, organic acids, and fatty acid derivatives. In addition, 25 different compounds were identified in the different batches of mulberry leaves. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity was measured to evaluate the in vitro antioxidant activities of mulberry leaves. Among the four batches, batch A, harvested in December, exhibited the strongest DPPH radical-scavenging activity, while batch B, harvested in March, showed the weakest activity. This was related to the total phenolic content in the mulberry leaves of each batch. The optimal harvest time of mulberry leaves greatly influences the bioactivity and bioavailability of the plant.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules28041947