Effects of gamma-aminobutyric acid and piperine on gene regulation in pig kidney epithelial cell lines

Gamma-aminobutyric acid (GABA) and piperine (PIP) are both nutritional supplements with potential use in animal diets. The purpose of this study is to investigate the effect of GABA and/or PIP treatment on the gene expression pattern of a pig kidney epithelial cell line. LLCPK1 cells were treated wi...

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Bibliographic Details
Published in:Animal bioscience 2020, 33(9), , pp.1497-1506
Main Authors: Shin, Juhyun, Lee, Yoon-Mi, Oh, Jeongheon, Jung, Seunghwa, Oh, Jae-Wook
Format: Article
Language:English
Subjects:
Dietary supplements
erythrocyte differentiation
GABA
gamma-aminobutyric acid
Genes
Genetic transcription
immune system
mapk pathway
microarray
pig kidney epithelial cell
piperine
Swine
Transcription (Genetics)
축산학
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Summary:Gamma-aminobutyric acid (GABA) and piperine (PIP) are both nutritional supplements with potential use in animal diets. The purpose of this study is to investigate the effect of GABA and/or PIP treatment on the gene expression pattern of a pig kidney epithelial cell line. LLCPK1 cells were treated with GABA, PIP, or both, and then the gene expression pattern was analyzed using microarray. Gene ontology analysis was done using GeneOntology (Geneontology.org), and validation was performed using quantitative RT-PCR. Gene ontology enrichment analysis was used to identify key pathway(s) of genes whose expression levels were regulated by these treatments. Microarray results showed that GABA had a positive effect on the transcription of genes related to regulation of erythrocyte differentiation and that GABA and PIP in combination had a synergistic effect on genes related to immune systems and processes. Furthermore, we found that effects of GABA and/or PIP on these selected genes were controlled by JNK/p38 MAPK pathway. These results can improve our understanding of mechanisms involved in the effect of GABA and/or PIP treatment on pig kidney epithelial cells. They can also help us evaluate their potential as a clinical diagnosis and treatment.
ISSN:1011-2367
2765-0189
1976-5517
2765-0235
DOI:10.5713/ajas.19.0745