A modified hydrostatic microfluidic pumpless device for in vitro murine ovarian tissue culture as research model for fertility preservation

• Published in: Obstetrics & gynecology science 2022-07, Vol.65 (4), p.376-381
• Main Authors: Thuwanut, Paweena, Pimpin, Alongkorn, Thatsanabunjong, Fueangrat, Srisuwatanasagul, Sayamon, Sereepapong, Wisan, Sirayapiwat, Porntip
• Format: Article
• Language: English
• Subjects: crofluidics; fertility preservation; ovarian follicle; Short Communication; technique
• ISSN: 2287-8572; 2287-8580
• DOI: 10.5468/ogs.22012

This study aimed to compare the efficacies of conventional and non-conventional (modified hydrostatic microfluidic pumpless device; MHPD) systems on ovarian tissue culture and in vitro follicle growth using a mouse model. A total of 56 ovarian cortical tissues retrieved from seven wild-type mice were divided into three groups: I) fresh control, II) conventional culture system (control), and II) non-conventional system with MHPD. Ovarian tissues were cultured for 96 hours and evaluated for follicle morphology, developmental stage, intact follicle density, and relative gene expression levels (PCNA, IGF-1, BAX, and Bcl-2). Our major data demonstrated that the mean percentage of primary follicle development was increased by the MHPD (P < 0.05). In addition, this device could maintain and support follicle development better than the conventional culture systems. However, the overall outcomes were not significantly improved by our first-design prototype. Consequently, next-generation platforms should be developed as alternative medical tools for fertility preservation research.