High-Throughput Identification of Organic Compounds from Polygoni Multiflori Radix Praeparata (Zhiheshouwu) by UHPLC-Q-Exactive Orbitrap-MS

Polygoni Multiflori Radix Praeparata (PMRP), as the processed product of tuberous roots of Polygonum multiflorum Thunb., is one of the most famous traditional Chinese medicines, with a long history. However, in recent years, liver adverse reactions linked to PMRP have been frequently reported. Our w...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2021-06, Vol.26 (13), p.3977
Main Authors: Wang, Shaoyun, Sun, Xiaozhu, An, Shuo, Sang, Fang, Zhao, Yunli, Yu, Zhiguo
Format: Article
Language:English
Subjects:
Anthraquinones
Aquatic plants
chemical constituents
Constituents
Ethanol
Flavones
Herbal medicine
High performance liquid chromatography
In vivo methods and tests
Lipids
Liquid chromatography
Liver
Mass spectrometry
Mass spectroscopy
Metabolism
Metabolites
Organic compounds
Phenolic acids
Phenols
polygoni multiflori radix praeparata
Quadrupoles
traditional Chinese medicine
UHPLC-Q-Exactive Orbitrap-MS
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Summary:Polygoni Multiflori Radix Praeparata (PMRP), as the processed product of tuberous roots of Polygonum multiflorum Thunb., is one of the most famous traditional Chinese medicines, with a long history. However, in recent years, liver adverse reactions linked to PMRP have been frequently reported. Our work attempted to investigate the chemical constituents of PMRP for clinical research and safe medication. In this study, an effective and rapid method was established to separate and characterize the constituents in PMRP by combining ultra-high performance liquid chromatography with hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS). Based on the accurate mass measurements for molecular and characteristic fragment ions, a total of 103 compounds, including 24 anthraquinones, 21 stilbenes, 15 phenolic acids, 14 flavones, and 29 other compounds were identified or tentatively characterized. Forty-eight compounds were tentatively characterized from PMRP for the first time, and their fragmentation behaviors were summarized. There were 101 components in PMRP ethanol extract (PMRPE) and 91 components in PMRP water extract (PMRPW). Simultaneously, the peak areas of several potential xenobiotic components were compared in the detection, which showed that PMRPE has a higher content of anthraquinones and stilbenes. The obtained results can be used in pharmacological and toxicological research and provided useful information for further in vitro and in vivo studies.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules26133977