ORYZA SATIVA SPOTTED-LEAF 41 (OsSPL41) Negatively Regulates Plant Immunity in Rice

• Published in: Rice science 2023-09, Vol.30 (5), p.426-436
• Main Authors: Jingyi, Tan, Xiaobo, Zhang, Huihui, Shang, Panpan, Li, Zhonghao, Wang, Xinwei, Liao, Xia, Xu, Shihua, Yang, Junyi, Gong, Jianli, Wu
• Format: Article
• Language: English
• Subjects: bacterial blight; leucine-rich repeat receptor-like protein kinase; plant immunity; reactive oxygen species; rice; spotted leaf
• ISSN: 1672-6308
• DOI: 10.1016/j.rsci.2023.02.004

Identification of immunity-associated leucine-rich repeat receptor-like protein kinases(LRR-RLK)is critical to elucidate the LRR-RLK mediated mechanism of plant immunity.Here,we reported the map-based cloning of a novel rice SPOTTED-LEAF 41(OsSPL41)encoding a putative LRR-RLK protein(OsLRR-RLK41/OsSPL41)that regulated disease responses to the bacterial blight pathogen Xanthomonas oryzae pv.oryzae(Xoo).An 8-bp insertion at position 865 bp in a mutant spotted-leaf 41(spl41)allele led to the formation of purple-brown lesions on leaves.Functional complementation by the wild type allele(OsSPL41)can rescue the mutant phenotype,and the complementary lines showed similar performance to wild type in a number of agronomic,physiological and molecular indices.OsSPL41 was constitutively expressed in all tissues tested,and OsSPL41 contains a typical transmembrane domain critical for its localization to the cell membrane.The mutant exhibited an enhanced level of resistance to Xoo in companion of markedly up-regulated expression of pathogenesis-related genes such as OsPR10a,OsPAL1 and OsNPR1,while the level of salicylic acid was significantly increased in spl41.In contrast,the over-expression lines exhibited a reduced level of H2O2 and were much susceptible to Xoo with down-regulated expression of pathogenesis-related genes.These results suggested that OsSPL41 might negatively regulate plant immunity through the salicylic acid signaling pathway in rice.