Regulation of Recombination between gtfB/gtfC Genes in Streptococcus mutans by Recombinase A

Streptococcus mutans produces 3 types of glucosyltransferases (GTFs), whose cooperative action is essential for cellular adhesion. The recombinase A (RecA) protein is required for homologous recombination. In our previous study, we isolated several strains with a smooth colony morphology and low GTF...

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Published in:TheScientificWorld 2013-01, Vol.2013 (2013), p.1-7
Main Authors: Matsumoto-Nakano, Michiyo, Matsumi, Yuki, Ardin, Arifah C., Nagayama, Kayoko, Takashima, Yukiko, Fujita, Kazuyo, Inagaki, Satoko
Format: Article
Language:English
Subjects:
Adhesion
Bacterial Adhesion
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacteriology
Base Sequence
Cell adhesion
Colonies
Dental caries
Dentistry
DNA, Bacterial - genetics
Enzyme Activation
Gene expression
Gene Expression Regulation, Bacterial
Gene Expression Regulation, Enzymologic
Gene Fusion
Gene sequencing
Genes
Genes, Bacterial
Genetic aspects
Genetic recombination
Genetic research
Homology
Microbiological research
Microbiology
Morphology
Pediatrics
Plasmids
Proteins
Rec A Recombinases - genetics
Rec A Recombinases - metabolism
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Recombination, Genetic
Sequence Analysis, DNA
Signal transduction
Streptococcus
Streptococcus infections
Streptococcus mutans
Streptococcus mutans - drug effects
Streptococcus mutans - enzymology
Streptococcus mutans - genetics
Stress response
Sucrose
Sucrose - pharmacology
University graduates
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cited_by cdi_FETCH-LOGICAL-c699t-59c8d999ad656b3bd5f5bf0213dcef9f53fc60d8c21acedac3dbf8bc738acf363
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container_title TheScientificWorld
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creator Matsumoto-Nakano, Michiyo
Matsumi, Yuki
Ardin, Arifah C.
Nagayama, Kayoko
Takashima, Yukiko
Fujita, Kazuyo
Inagaki, Satoko
description Streptococcus mutans produces 3 types of glucosyltransferases (GTFs), whose cooperative action is essential for cellular adhesion. The recombinase A (RecA) protein is required for homologous recombination. In our previous study, we isolated several strains with a smooth colony morphology and low GTF activity, characteristics speculated to be derived from the GTF fusions. The purpose of the present study was to investigate the mechanism of those fusions. S. mutans strain MT8148 was grown in the presence of recombinant RecA (rRecA) protein, after which smooth colonies were isolated. The biological functions and sequences of the gtfB and gtfC genes of this as well as other clinical strains were determined. The sucrose-dependent adherence rates of those strains were reduced as compared to that of MT8148. Determination of the sequences of the gtfB and gtfC genes showed that an approximately 3500 bp region was deleted from the area between them. Furthermore, expression of the recA gene was elevated in those strains as compared to MT8148. These results suggest that RecA has an important role in fusions of gtfB and gtfC genes, leading to alteration of colony morphology and reduction in sucrose-dependent adhesion.
doi_str_mv 10.1155/2013/405075
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subjects Adhesion
Bacterial Adhesion
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacteriology
Base Sequence
Cell adhesion
Colonies
Dental caries
Dentistry
DNA, Bacterial - genetics
Enzyme Activation
Gene expression
Gene Expression Regulation, Bacterial
Gene Expression Regulation, Enzymologic
Gene Fusion
Gene sequencing
Genes
Genes, Bacterial
Genetic aspects
Genetic recombination
Genetic research
Homology
Microbiological research
Microbiology
Morphology
Pediatrics
Plasmids
Proteins
Rec A Recombinases - genetics
Rec A Recombinases - metabolism
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Recombination, Genetic
Sequence Analysis, DNA
Signal transduction
Streptococcus
Streptococcus infections
Streptococcus mutans
Streptococcus mutans - drug effects
Streptococcus mutans - enzymology
Streptococcus mutans - genetics
Stress response
Sucrose
Sucrose - pharmacology
University graduates
title Regulation of Recombination between gtfB/gtfC Genes in Streptococcus mutans by Recombinase A
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