Titanium Dioxide Nanoparticles Negatively Influence Gill Metabolism in Pinctada fucata martensii

• Published in: Metabolites 2024-12, Vol.14 (12), p.682
• Main Authors: Zou, Heqi, Li, Fengfeng, Huang, Luomin, Yao, Jiaying, Lin, Yujing, Yang, Chuangye, Hao, Ruijuan, Mkuye, Robert, Liao, Yongshan, Deng, Yuewen
• Format: Article
• Language: English
• Subjects: Acetylcholine; Adenosine triphosphatase; Amino acids; Analysis; Aquatic animals; Arginine; Bivalvia; Ca2+/Mg2+-exchanging ATPase; Calcium metabolism; Carnitine; Chemical properties; Chromatography; Citric acid; Energy metabolism; Gas flow; Gills; Glucosamine; Glutathione; Hepatopancreas; Isocitric acid; Itaconic acid; Levocarnitine; Magnesium; Mass spectrometry; Metabolism; Metabolites; Metabolomics; N-Acetylglucosamine; Na+/K+-exchanging ATPase; Nanoparticles; Neurotoxicity; Organisms; Osmoregulation; Oxidative metabolism; Oxidative stress; Oysters; Pentose phosphate pathway; Pinctada fucata martensii; Pinctada fucata martensii; Scientific imaging; Sea-water; Seawater; Software; Titanium dioxide; titanium dioxide nanoparticles; Toxicity; Tricarboxylic acid cycle
• ISSN: 2218-1989; 2218-1989
• DOI: 10.3390/metabo14120682

In recent years, titanium dioxide (TiO ) nanoparticles (NPs) have been widely used in various industries due to their favorable chemical properties, and their contamination of the environment has attracted much attention, especially to aquatic animals. Therefore, we assessed the impact of TiO NPs (5 mg/L) on the marine bivalve, pearl oyster ( ), especially gill metabolism. Pearl oysters were exposed to seawater containing 5 mg/L TiO NPs for 14 days, followed by 7 days of recovery in untreated seawater. Gill tissues and hepatopancreatic tissues were sampled on days 0, 14, and 21 of the experiment named C0, E14, and R7, respectively. Metabolomic analysis identified 102 significantly different metabolites (SDMs) on gills tissue in pearl oysters following exposure to TiO NPs (C0 vs. E14). Compared with group C0, group E14 had 76 SDMs (such as acetylcholine, itaconic acid, citric acid, and taurine) with higher concentrations and 26 (including L-arginine and isobutyryl-L-carnitine) with lower concentrations. KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis showed that these SDMs enriched 28 pathways, including glycine, serine, and threonine metabolism, neuroactive ligand-receptor interaction, and taurine and hypotaurine metabolism. In addition, 116 SDMs were identified in E14 and R7 pearl oysters. Compared with group E14, group R7 had 74 metabolites (such as acetylcholine, 6-phosphogluconic acid, isocitric acid, and itaconic acid) with higher concentrations and 42 (including uracil, glycerophosphocholine, N-Acetyl-D-glucosamine) with lower concentrations. The SDMs identified between E14 and R7 enriched 25 pathways, including the pentose phosphate pathway, glutathione metabolism, and citrate cycle (TCA cycle). In addition, analysis of the energy metabolism-associated enzymes revealed that exposure to TiO NPs reduced Ca /Mg -ATPase, Na /K -ATPase, and Total-ATPase activities. These findings suggested that TiO NPs may inhibit the energy metabolism function of gill and hepatopancreas of pearl oysters. Meanwhile, TiO NPs may affect the normal functioning of immune and osmoregulatory functions of pearl oysters gill and even may lead to oxidative stress and neurotoxicity. Therefore, this study may provide a reference for analyzing the bioadaptation of marine bivalves to TiO NPs and the potential negative effects of TiO NPs on bivalves.