Molecular mechanism of interleukin-17A regulating airway epithelial cell ferroptosis based on allergic asthma airway inflammation

• Published in: Redox biology 2023-12, Vol.68, p.102970, Article 102970
• Main Authors: Song, Jingjing, Zhang, Hui, Tong, Yu, Wang, Yufei, Xiang, Qiangwei, Dai, Huan, Weng, Cuiye, Wang, Lei, Fan, Junwen, Shuai, Yilong, Lai, Chuqiao, Fang, Xiaoxiao, Chen, Mingxin, Bao, Jiali, Zhang, Weixi
• Format: Article
• Language: English
• Subjects: Airway inflammation; Animals; Asthma; Asthma - pathology; Bronchial epithelial cells; Disease Models, Animal; Epithelial Cells - metabolism; Ferroptosis; Humans; Inflammation - genetics; Interleukin-17 - adverse effects; Interleukin-17 - genetics; Interleukin-17 - metabolism; Interleukin-17A; Lung - metabolism; Mice; Mice, Inbred BALB C; Ovalbumin - adverse effects; Research Paper
• ISSN: 2213-2317; 2213-2317
• DOI: 10.1016/j.redox.2023.102970

Interleukin-17A (IL-17A) levels are elevated in patients with asthma. Ferroptosis has been identified as the non-apoptotic cell death type associated with asthma. Data regarding the relation of ferroptosis with asthma and the effect of IL-17A on modulating ferroptosis in asthma remain largely unclear. The present work focused on investigating the role of IL-17A in allergic asthma-related ferroptosis and its associated molecular mechanisms using public datasets, clinical samples, human bronchial epithelial cells, and an allergic asthma mouse model. We found that IL-17A was significantly upregulated within serum in asthma cases. Adding IL-17A significantly increased ferroptosis within human bronchial epithelial cells (BEAS-2B). In ovalbumin (OVA)-induced allergic asthmatic mice, IL-17A regulated and activated lipid peroxidation induced ferroptosis, whereas IL-17A knockdown effectively inhibited ferroptosis in vivo by protection of airway epithelial cells via the xCT-GSH-GPX4 antioxidant system and reduced airway inflammation. Mouse mRNA sequencing results indicated that the tumor necrosis factor (TNF) pathway was the differential KEGG pathway in the OVA group compared to healthy controls and the OVA group compared to the IL-17A knockout OVA group. We further used N-acetylcysteine (TNF inhibitor) to inhibit the TNF signaling pathway, which was found to protect BEAS-2B cells from IL-17A induced lipid peroxidation and ferroptosis damage. Our findings reveal a novel mechanism for the suppression of ferroptosis in airway epithelial cells, which may represent a new strategy for the use of IL-17A inhibitors against allergic asthma.