Effect of fumigation height and time on cryopreservation of ram semen

The cooling rate is a crucial factor in the process of freezing semen, influencing the overall freezing effectiveness. The height and time of fumigation can significantly impact the rate of cooling. Appropriate cooling rates can help minimize the formation of ice crystals in spermatozoa and reduce p...

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Bibliographic Details
Published in:Scientific reports 2024-05, Vol.14 (1), p.10944-10944, Article 10944
Main Authors: Zhang, Liuming, Wang, Xuyang, Jiang, Caiyu, Sun, Yuxuan, Sohail, Tariq, Sun, Xiaomei, Wang, Jian, Li, Yongjun
Format: Article
Language:English
Subjects:
631/601
631/601/1737
Acrosome - drug effects
Animals
Cell Membrane - drug effects
Cooling
Cryopreservation
Cryopreservation - methods
Crystals
Freezing
Fumigation
Fumigation - methods
Fumigation height and time
Functional integrity
Humanities and Social Sciences
Ice
Male
Motility
Motility parameters
multidisciplinary
Ram
Reactive oxygen species
Reactive Oxygen Species - metabolism
ROS
Science
Science (multidisciplinary)
Semen
Semen - drug effects
Semen Preservation - methods
Sheep
Sperm
Sperm Motility - drug effects
Spermatozoa
Spermatozoa - drug effects
Spermatozoa - physiology
Time Factors
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Summary:The cooling rate is a crucial factor in the process of freezing semen, influencing the overall freezing effectiveness. The height and time of fumigation can significantly impact the rate of cooling. Appropriate cooling rates can help minimize the formation of ice crystals in spermatozoa and reduce potential damage to them. Therefore, the aim of this study was to evaluate the effect of different fumigation heights and time for the cryopreservation of Hu ram semen. Experiments I–IV assessed the effect of semen cryopreservation by testing the post-thawed spermatozoa total motility (TM), progressive motility (PM) and kinetic parameters fumigated at distances of 2, 4, 6 and 8 cm for durations of 5, 10, 15 and 20 min, respectively. Based on the results of experiments I to IV, experiment V evaluated the effect of semen cryopreservation by testing the post-thawed spermatozoa TM, PM, kinetic parameters, plasma membrane integrity, acrosome integrity and reactive oxygen species (ROS) level fumigated at distances of 2, 4, 6 and 8 cm for duration of 20 min. The results indicated that fumigation at 2 cm for 20 min significantly ( P  
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-61947-x