Microbial Species Coexistence Depends on the Host Environment

• Published in: mBio 2020-07, Vol.11 (4)
• Main Authors: Deines, Peter, Hammerschmidt, Katrin, Bosch, Thomas C G
• Format: Article
• Language: English
• Subjects: Host-Microbe Biology; host-microbe interactions; Hydra; metaorganism; microbiome; species coexistence
• ISSN: 2161-2129; 2150-7511
• DOI: 10.1128/mBio.00807-20

Organisms and their resident microbial communities form a complex and mostly stable ecosystem. It is known that the specific composition and abundance of certain bacterial species affect host health and fitness, but the processes that lead to these microbial patterns are unknown. We investigate this by deconstructing the simple microbiome of the freshwater polyp We contrast the performance of its two main bacterial associates, and , on germfree hosts with two environments over time. We show that interactions within the microbiome but also the host environment lead to the observed species frequencies and abundances. More specifically, we find that both microbial species can only stably coexist in the host environment, whereas outcompetes in both environments irrespective of initial starting frequencies. While seems to benefit through secretions of , its competitive effect on depends upon direct contact. The competition might potentially be mitigated through the spatial distribution of the two microbial species on the host, which would explain why both species stably coexist on the host. Interestingly, the relative abundances of both species on the host do not match the relative abundances reported previously nor the overall microbiome carrying capacity as reported in this study. Both observations indicate that rare microbial community members might be relevant for achieving the native community composition and carrying capacity. Our study highlights that for dissecting microbial interactions the specific environmental conditions need to be replicated, a goal difficult to achieve with systems. This work studies microbial interactions within the microbiome of the simple cnidarian and investigates whether microbial species coexistence and community stability depend on the host environment. We find that the outcome of the interaction between the two most dominant bacterial species in 's microbiome differs depending on the environment and results in a stable coexistence only in the host context. The interactive ecology between the host and the two most dominant microbes, but also the less abundant members of the microbiome, is critically important for achieving the native community composition. This indicates that the metaorganism environment needs to be taken into account when studying microbial interactions.