Generation of a lamin A/C knockout human induced pluripotent stem cell line (ZJULLi007-A) via CRISPR/Cas9

Lamin A/C is a protein encoded by the LMNA gene and belongs to the nuclear lamina protein family. Mutations in the LMNA gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth diseas...

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Bibliographic Details
Published in:Stem cell research 2024-12, Vol.81, p.103579, Article 103579
Main Authors: Liu, Dandan, Shen, Jiaxi, Yang, Zongkuai, Fan, Hangping, Wang, Hao, Liang, Ping, Gong, Tingyu
Format: Article
Language:English
Subjects:
Cell Line
CRISPR-Cas Systems
Gene Editing
Gene Knockout Techniques
Humans
Induced Pluripotent Stem Cells - metabolism
Lamin Type A - genetics
Lamin Type A - metabolism
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Summary:Lamin A/C is a protein encoded by the LMNA gene and belongs to the nuclear lamina protein family. Mutations in the LMNA gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. In this study, a lamin A/C knockout human induced pluripotent stem cell line was successfully generated using the CRISPR/Cas9 genome-editing technology, which was confirmed with normal pluripotency and karyotype.
ISSN:1873-5061
1876-7753
1876-7753
DOI:10.1016/j.scr.2024.103579