High-Resolution Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy of Paired Clinical Liver Tissue Samples from Hepatocellular Cancer and Surrounding Region

The global burden of liver cancer is increasing. Timely diagnosis is important for optimising the limited available treatment options. Understanding the metabolic consequences of hepatocellular carcinoma (HCC) may lead to more effective treatment options. We aimed to document metabolite differences...

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Bibliographic Details
Published in:International journal of molecular sciences 2024-08, Vol.25 (16), p.8924
Main Authors: Fernandes, Wendy M, Harris, Nicola, Zamalloa, Ane, Adofina, Lissette, Srinivasan, Parthi, Menon, Krishna, Heaton, Nigel, Miquel, Rosa, Zen, Yoh, Kelly, Geoff, Jarvis, James A, Oregioni, Alain, Chokshi, Shilpa, Riva, Antonio, Cox, I Jane
Format: Article
Language:English
Subjects:
Adult
Aged
bacterial metabolites
Carcinoma, Hepatocellular - metabolism
Carcinoma, Hepatocellular - pathology
Female
hepatocellular carcinoma
Humans
Lipid Metabolism
Lipids
Lipids - analysis
Liver - metabolism
Liver - pathology
Liver cancer
Liver Neoplasms - metabolism
Liver Neoplasms - pathology
Magnetic Resonance Spectroscopy - methods
Male
Metabolites
Metabolome
Middle Aged
NMR
NMR spectroscopy
Nuclear magnetic resonance
personalised treatment
Spectrum analysis
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Summary:The global burden of liver cancer is increasing. Timely diagnosis is important for optimising the limited available treatment options. Understanding the metabolic consequences of hepatocellular carcinoma (HCC) may lead to more effective treatment options. We aimed to document metabolite differences between HCC and matched surrounding tissues of varying aetiology, obtained at the time of liver resection, and to interpret metabolite changes with clinical findings. High-resolution magic angle spinning nuclear magnetic resonance (HRMAS-NMR) spectroscopy analyses of N = 10 paired HCC and surrounding non-tumour liver tissue samples were undertaken. There were marked HRMAS-NMR differences in lipid levels in HCC tissue compared to matched surrounding tissue and more subtle changes in low-molecular-weight metabolites, particularly when adjusting for patient-specific variability. Differences in lipid-CH , lipid-CH , formate, and acetate levels were of particular interest. The obvious differences in lipid content highlight the intricate interplay between metabolic adaptations and cancer cell survival in the complex microenvironment of liver cancer. Differences in formate and acetate might relate to bacterial metabolites. Therefore, documentation of metabolites in HCC tissue according to histology findings in patients is of interest for personalised medicine approaches and for tailoring targeted treatment strategies.
ISSN:1661-6596
1422-0067
1422-0067
DOI:10.3390/ijms25168924