The Skeletal Muscle, the Heart, and the Liver Are the Major Organs of the Accumulation of Nitric Oxide Metabolites after Oral Nitrite Treatment

Nitrite is a nitric oxide (NO) metabolite, which may be bioactivated to generate NO in vivo and supplement endogenous NO formation, especially in cardiovascular and metabolic diseases. However, it is not known whether treatment with oral nitrite results in the accumulation of NO metabolites in diffe...

Full description

Saved in:
Bibliographic Details
Published in:Antioxidants 2024-02, Vol.13 (3), p.255
Main Authors: Lima-Silva, Ana K, Rebelo, Macario A, Barros, Alessandra C, Conde-Tella, Sandra O, Tanus-Santos, Jose E
Format: Article
Language:English
Subjects:
Aorta
Cardiac muscle
cardiovascular diseases
Chemiluminescence
Coronary vessels
Enzymes
Heart
Laboratory animals
Liver
Metabolic disorders
Metabolites
Muscles
Nitrates
Nitric oxide
nitric oxide metabolites
Nitrites
Nitrogen
Omeprazole
Oral administration
Physiology
Plasma
Skeletal muscle
Sodium nitrite
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nitrite is a nitric oxide (NO) metabolite, which may be bioactivated to generate NO in vivo and supplement endogenous NO formation, especially in cardiovascular and metabolic diseases. However, it is not known whether treatment with oral nitrite results in the accumulation of NO metabolites in different organs. Moreover, treatment with omeprazole, an inhibitor of gastric acid secretion, severely affects the gastric formation of S-nitrosothiols induced with oral nitrite treatment. However, no previous study has examined whether omeprazole affects the nitrite-induced accumulation of NO metabolites in different organs. This study examined in rats the effects of oral sodium nitrite treatment (15 mg/kg via gavage for 1 or 7 days) associated with omeprazole (10 mg/kg or vehicle) on nitrite and nitrate and nitrosylated species (RXNO) concentrations (measured using ozone-based chemiluminescence methods) assessed in the plasma, aorta, heart, liver, brain, and muscle. While our results showed that NO metabolite accumulation in different organs is not uniform, we found that the skeletal muscle, the heart, and the liver accumulate NO metabolites, particularly RXNO. This response was significantly attenuated by omeprazole in the heart and in the skeletal muscle. Together, these findings may indicate that the skeletal muscle, the heart, and the liver are major reservoir sites for NO metabolites after oral nitrite treatment, with major increases in nitrosylated species.
ISSN:2076-3921
2076-3921
DOI:10.3390/antiox13030255