Regulation of Adipogenesis and Lipid Deposits by Collapsin Response Mediator Protein 2

• Published in: International journal of molecular sciences 2020-03, Vol.21 (6), p.2172
• Main Authors: Chang, Yih-Hsin, Tsai, Jen-Ning, Chang, Shu-Wen, Hsu, Wei-Ting, Yang, Ching-Ping, Hsiao, Chiao-Wan, Shiau, Ming-Yuh
• Format: Article
• Language: English
• Subjects: 3T3-L1 Cells; Abnormalities; Actin; Actins - metabolism; Adipocytes; Adipocytes - cytology; Adipocytes - metabolism; Adipogenesis; Adipogenesis - genetics; Animals; Axonogenesis; Cell growth; Cell proliferation; Cell Proliferation - genetics; Collapsin response mediator protein 2; Cyclin-dependent kinases; Cytoskeleton; Cytoskeleton - metabolism; Deposits; Diabetes; Diet, High-Fat; Energy; Energy balance; Enzymes; Gene Knockdown Techniques; Gene Silencing; Glucose - metabolism; Glucose Transporter Type 4 - metabolism; Glycogen Synthase Kinase 3 beta - metabolism; glycogen synthase kinase-3β; Homeostasis; In vivo methods and tests; Insulin resistance; Intercellular Signaling Peptides and Proteins - genetics; Intercellular Signaling Peptides and Proteins - metabolism; Kinases; Lipid metabolism; Lipid Metabolism - genetics; Lipids; Male; Mediator protein; Metabolic disorders; Metabolism; Mice; Mice, Inbred C57BL; Nerve Tissue Proteins - genetics; Nerve Tissue Proteins - metabolism; neurodegenerative disease; Obesity; Obesity - genetics; Obesity - metabolism; Phosphatase; Phosphorylation; Polymerization; Proteins; RNA, Small Interfering; Signal Transduction - genetics; Transcription factors; Up-Regulation
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21062172

As emerging evidence suggesting neurodegenerative diseases and metabolic diseases have common pathogenesis, we hypothesized that the neurite outgrowth-controlling collapsin response mediator protein 2 (CRMP2) was involved in energy homeostasis. Therefore, putative roles of CRMP2 in adipocyte differentiation (adipogenesis) and lipid metabolism were explored and addressed in this study. CRMP2 expression profiles were in vitro and in vivo characterized during adipogenic process of 3T3-L1 pre-adipocytes and diet-induced obese (DIO) mice, respectively. Effects of CRMP2 on lipid metabolism and deposits were also analyzed. Our data revealed that CRMP2 expression pattern was coupled with adipogenic stages. CRMP2 overexpression inhibited cell proliferation at MCE phase, and significantly reduced lipid contents by down-regulating adipogenesis-driving transcription factors and lipid-synthesizing enzymes. Interestingly, GLUT4 translocation and the lipid droplets fusion were disturbed in CRMP2-silencing cells by affecting actin polymerization. Moreover, adipose CRMP2 was significantly increased in DIO mice, indicating CRMP2 is associated with obesity. Accordingly, CRMP2 exerts multiple functions in adipogenesis and lipid deposits through mediating cell proliferation, glucose/lipid metabolism and cytoskeleton dynamics. The present study identifies novel roles of CRMP2 in mediating adipogenesis and possible implication in metabolic disorders, as well as provides molecular evidence supporting the link of pathogenesis between neurodegenerative diseases and metabolic abnormalities.