Proteomic Identification of an Endogenous Synaptic SUMOylome in the Developing Rat Brain

Synapses are highly specialized structures that interconnect neurons to form functional networks dedicated to neuronal communication. During brain development, synapses undergo activity-dependent rearrangements leading to both structural and functional changes. Many molecular processes are involved...

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Bibliographic Details
Published in:Frontiers in molecular neuroscience 2021-11, Vol.14, p.780535-780535
Main Authors: Pronot, Marie, Kieffer, Félicie, Gay, Anne-Sophie, Debayle, Delphine, quet, Raphaël, Poupon, Gwénola, Schorova, Lenka, Martin, Stéphane, Gwizdek, Carole
Format: Article
Language:English
Subjects:
Binding sites
Biochemistry
Biochemistry, Molecular Biology
Brain
Cell Behavior
Cellular Biology
Cognitive science
Cytoskeleton
Enzymes
Immunoprecipitation
Life Sciences
Mass spectroscopy
Monoclonal antibodies
Neuroscience
Neurotransmitter receptors
Post-translation
post-translational modification
Proteins
Proteomes
proteomics
Structure-function relationships
Sucrose
SUMO
SUMOylome
synapse
Synaptogenesis
Ubiquitin
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Summary:Synapses are highly specialized structures that interconnect neurons to form functional networks dedicated to neuronal communication. During brain development, synapses undergo activity-dependent rearrangements leading to both structural and functional changes. Many molecular processes are involved in this regulation, including post-translational modifications by the Small Ubiquitin-like MOdifier SUMO. To get a wider view of the panel of endogenous synaptic SUMO-modified proteins in the mammalian brain, we combined subcellular fractionation of rat brains at the post-natal day 14 with denaturing immunoprecipitation using SUMO2/3 antibodies and tandem mass spectrometry analysis. Our screening identified 803 candidate SUMO2/3 targets, which represents about 18% of the synaptic proteome. Our dataset includes neurotransmitter receptors, transporters, adhesion molecules, scaffolding proteins as well as vesicular trafficking and cytoskeleton-associated proteins, defining SUMO2/3 as a central regulator of the synaptic organization and function.
ISSN:1662-5099
1662-5099
DOI:10.3389/fnmol.2021.780535