Generation of an induced pluripotent stem cell line (ZZUi020-A) from a patient with Parkinson’s disease harboring the intermediate-length GGC repeat expansions in the NOTCH2NLC gene

Here, we describe the generation of an induced pluripotent stem cell (iPSC) line, from a female patient diagnosed with Parkinson’s disease (PD). The patient carries a heterozygous intermediate-length GGC repeat expansions mutation in the NOTCH2NLC gene. Skin fibroblasts were reprogrammed using the n...

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Bibliographic Details
Published in:Stem cell research 2021-04, Vol.52, p.102257-102257, Article 102257
Main Authors: Fan, Yu, Liu, Fen, Fan, Li-yuan, Mao, Cheng-yuan, Liu, Han, Zhang, Chan, Yuan, Yan-peng, Yang, Jing, Wang, Yan-lin, Liu, Yu-tao, Shi, Chang-he, Xu, Yu-ming
Format: Article
Language:English
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Summary:Here, we describe the generation of an induced pluripotent stem cell (iPSC) line, from a female patient diagnosed with Parkinson’s disease (PD). The patient carries a heterozygous intermediate-length GGC repeat expansions mutation in the NOTCH2NLC gene. Skin fibroblasts were reprogrammed using the non-integrating Sendai virus technology to deliver Klf4, OCT3/4, SOX2 and c-MYC factors. The generated iPSC line (ZZUi020-A) presented with expression of common pluripotency markers, showed potential of differentiating into derivatives of the three germ layers, and displayed a normal karyotype. The clone ZZUi020-A is presented thereafter, it can be used to study the mechanisms underlying NOTCH2NLC-PD pathogenesis.
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2021.102257