Evaluation of Guided Bone Regeneration in Critical Defects Using Bovine and Porcine Collagen Membranes: Histomorphometric and Immunohistochemical Analyses

Guided bone regeneration (GBR) is a technique used to facilitate bone regeneration, which uses a biocompatible membrane acting as a physical barrier to prevent the adjacent connective tissue from invading the bone defect. The aim of this study was to evaluate and compare the effectiveness of bovine...

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Bibliographic Details
Published in:International journal of biomaterials 2021, Vol.2021, p.8828194-9
Main Authors: Ramires, Guilherme A. D., Helena, Julia Taino, Oliveira, Júlio C. S. De, Faverani, Leonardo Perez, Bassi, Ana Paula F.
Format: Article
Language:English
Subjects:
Analysis
Animals
Antibodies
Barriers
BCG
BCG vaccines
Biocompatibility
Biomedical materials
Bone growth
Bones
Calvaria
Collagen
Connective tissues
Defects
Dentistry
Ethylenediaminetetraacetic acid
Immunohistochemistry
Laboratories
Membranes
Penicillin G benzathine
Physiology
Polylactic acid
Povidone
Regeneration
Regeneration (physiology)
Repair
Transplants & implants
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Summary:Guided bone regeneration (GBR) is a technique used to facilitate bone regeneration, which uses a biocompatible membrane acting as a physical barrier to prevent the adjacent connective tissue from invading the bone defect. The aim of this study was to evaluate and compare the effectiveness of bovine and porcine collagenous membranes as barriers to connective tissue invasion during the repair of critical bone defects in rat calvaria, using histological, histometric, and immunohistochemical analyses. For this study, 72 rats were divided into three groups: clot group (CG), bovine collagen group (BCG), and porcine collagen group (PCG). Analyses were performed on days 7, 15, 30, and 60. The histological results showed that the PCG exhibited bone neoformation starting from day 7, and after 30 days of repair, the surgical defect was completely filled in some animals. For the BCG, there was little bone neoformation activity in the initial periods, and from day 30 onwards, there was an increase in bone neoformation, with a greater increase on day 60. The data obtained in the histometric analysis reveal that, on day 30, the neoformed bone area did not vary greatly between the PCG and the BCG, though both varied from the CG. By day 60, the PCG presented a greater area of neoformation than the BCG. These results were corroborated by the immunohistochemistry results. In view of the results obtained, it can be concluded that all membranes studied in this research promoted GBR.
ISSN:1687-8787
1687-8795
DOI:10.1155/2021/8828194