Multi‐Functional Bio‐HJzyme: Revolutionizing Diabetic Skin Regeneration with its Glucose‐Unlocked Sterilization and Programmed Anti‐Inflammatory Effects

• Published in: Advanced science 2023-07, Vol.10 (21), p.e2300986-n/a
• Main Authors: He, Miaomiao, Wang, Zuyao, Yang, Hang, Wang, Qiancun, Xiang, Danni, Pang, Xinyan, Chan, Yau Kei, Sun, Dan, Yin, Guangfu, Yang, Weizhong, Deng, Yi
• Format: Article
• Language: English
• Subjects: Anti-Inflammatory Agents; antibacterial; Antioxidants; anti‐inflammation; bio‐heterojunction; cutaneous regeneration; Diabetes; Diabetes Mellitus; Efficiency; Enzymes; Glucose; Glucose Oxidase; glucose‐unlocked; Humans; Hyperglycemia; Infections; Inflammation; Light; Oxidation; Oxygen; Pathogens; Reactive Oxygen Species; Spectrum analysis; Sterilization; Wound Healing
• ISSN: 2198-3844; 2198-3844
• DOI: 10.1002/advs.202300986

Antibacterial dynamic therapy (ADT) triggered by reactive oxygen species (ROS) is promising for diabetic infectious disease treatment. However, the limited local O2/H2O2 production and post‐treatment inflammation remain long‐standing issues. To address these challenges, a novel H2‐evolving bio‐heterojunction enzyme (Bio‐HJzyme) consisting of graphite‐phase carbon nitride/copper sulfide (CN/Cu2−xS) heterojunction and glucose oxidase (GOx) is created. The Bio‐HJzyme offers glutathione peroxidase (GPx), peroxidase (POD), and catalase (CAT) mimetic activities; provides anti‐pathogen properties via programmed light activation; and effectively promotes diabetic wound healing. Specifically, its GPx‐mimetic activity and the presence of GOx significantly enhance the yield of H2O2, which can be catalyzed through POD‐mimetic activity to produce highly germicidal •OH. The H2O2 can also be catalyzed to H2O and O2, assisted by the CAT‐mimetic activity. The catalyzed products can then be catalyzed into germicidal •OH and •O2− under NIR light irradiation, giving enhanced ADT. Further, CN can split water to form H2 under solar light, which dramatically suppresses the inflammation caused by excessive ROS. In vivo evaluation confirms that Bio‐HJzyme promotes the regeneration of diabetic infectious skin through killing bacteria, enhancing angiogenesis, promoting wound bed epithelialization, and reinforcing anti‐inflammatory responses; hence, providing a revolutionary approach for diabetic wounds healing. A Bio‐HJzyme in terms of diabetic infectious wound regeneration is demonstrated, which works by glutathione peroxidase (GPx)‐, peroxidase (POD)‐, and catalase (CAT)‐mimetic activity for robust anti‐pathogenic property with the assistance of near‐infrared light as well as H2‐evolving anti‐inflammation upon solar light. A programmed‐light induced Bio‐HJzyme system for diabetic cutaneous regeneration is achieved.