Transcriptomic changes in oligodendrocyte lineage cells during the juvenile to adult transition in the mouse corpus callosum

• Published in: Scientific reports 2024-09, Vol.14 (1), p.22334-9, Article 22334
• Main Authors: Hoshino, Tomonori, Takase, Hajime, Hamanaka, Gen, Kimura, Shintaro, Fukuda, Norito, Mandeville, Emiri T., Lok, Josephine, Lo, Eng H., Arai, Ken
• Format: Article
• Language: English
• Subjects: 631/114/2164; 631/114/2403; 631/136/7; 631/378/2571; 631/378/2596; 631/378/2611; Age Factors; Aging; Aging - genetics; Animals; Cell Lineage - genetics; Corpus callosum; Corpus Callosum - cytology; Corpus Callosum - metabolism; Dementia disorders; Development; Gene expression; Gene Expression Profiling; Glial stem cells; Humanities and Social Sciences; Male; Mice; Mice, Inbred C57BL; multidisciplinary; Neurological diseases; Oligodendrocyte Precursor Cells - cytology; Oligodendrocyte Precursor Cells - metabolism; Oligodendrocytes; Oligodendroglia - cytology; Oligodendroglia - metabolism; RNA-sequencing (RNA-seq); Science; Science (multidisciplinary); Substantia alba; Transcriptome; Transcriptomics
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-72311-4

The corpus callosum, a major white matter tract in the brain, undergoes age-related functional changes. To extend our investigation of age-related gene expression dynamics in the mouse corpus callosum, we compared RNA-seq data from 2 week-old and 12 week-old wild-type C57BL/6 J mice and identified the differentially expressed genes (e.g., Marcksl1 , Chst3 , C4b , Neat1 , Ndrg1 , Emid1 , etc.) between these ages. Interestingly, we found that genes highly expressed in myelinating oligodendrocytes were upregulated in 12 week-old mice compared to 2 week-old mice, while genes highly expressed in oligodendrocyte precursor cells (OPCs) and newly formed oligodendrocytes were downregulated. Furthermore, by comparing these genes with the datasets from 20 week-old and 96 week-old mice, we identified novel sets of genes with age-dependent variations in the corpus callosum. These gene expression changes potentially affect key biological pathways and may be closely linked to age-related neurological disorders, including dementia and stroke. Therefore, our results provide an additional dataset to explore age-dependent gene expression dynamics of oligodendrocyte lineage cells in the corpus callosum.