Development of Paper-Based Fluorescent Molecularly Imprinted Polymer Sensor for Rapid Detection of Lumpy Skin Disease Virus

Lumpy Skin Disease (LSD) is a notifiable viral disease caused by Lumpy Skin Disease virus (LSDV). It is usually associated with high economic losses, including a loss of productivity, infertility, and death. LSDV shares genetic and antigenic similarities with Sheep pox virus (SPV) and Goat pox (GPV)...

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Published in:Molecules (Basel, Switzerland) Switzerland), 2024-04, Vol.29 (7), p.1676
Main Authors: Kassem, Samr, Hamdy, Mervat E, Selim, Karim M, Elmasry, Dalia M A, Shahein, Momtaz A, El-Husseini, Dalia M
Format: Article
Language:English
Subjects:
Animals
Antibodies
Biocompatibility
biosensor
Biosensors
Cattle
Coloring Agents
Cost analysis
Cross Reactions
Enzymes
fluorescent
Foot & mouth disease
Head
Infectious diseases
Influenza
LSDV
Lumpy skin disease virus
MIPs
Molecularly Imprinted Polymers
Nanomaterials
Nanoparticles
paper-based
Polymerization
Polymers
Quantum dots
Sensors
Sheep
Skin
Skin diseases
Viruses
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Summary:Lumpy Skin Disease (LSD) is a notifiable viral disease caused by Lumpy Skin Disease virus (LSDV). It is usually associated with high economic losses, including a loss of productivity, infertility, and death. LSDV shares genetic and antigenic similarities with Sheep pox virus (SPV) and Goat pox (GPV) virus. Hence, the LSDV traditional diagnostic tools faced many limitations regarding sensitivity, specificity, and cross-reactivity. Herein, we fabricated a paper-based turn-on fluorescent Molecularly Imprinted Polymer (MIP) sensor for the rapid detection of LSDV. The LSDV-MIPs sensor showed strong fluorescent intensity signal enhancement in response to the presence of the virus within minutes. Our sensor showed a limit of detection of 10 log TCID50/mL. Moreover, it showed significantly higher specificity to LSDV relative to other viruses, especially SPV. To our knowledge, this is the first record of a paper-based rapid detection test for LSDV depending on fluorescent turn-on behavior.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules29071676