Isolation and determination of biochemical nature of water soluble anticoagulant from earthworm

Earthworms are commonly known as farmer’s friend. Various previous studies have confirmed the anti-inflammatory, analgesic, antipyretic and anticancer effects of earthworm extract. In the present study selected species of earthworm was used to study the anticoagulative activity of earthworm extract...

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Bibliographic Details
Published in:Environment conservation journal 2011-06, Vol.12 (1&2), p.75-77
Main Authors: Mathur, Abhishek, Verma, Satish K., Singh, Santosh K., Prakash, Archana, Prasad, G.B.K.S., Dua, V.K.
Format: Article
Language:English
Subjects:
Anticoagulant activity
APTT
DNA
Eudrilus eugeniae
Hydrolases
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Summary:Earthworms are commonly known as farmer’s friend. Various previous studies have confirmed the anti-inflammatory, analgesic, antipyretic and anticancer effects of earthworm extract. In the present study selected species of earthworm was used to study the anticoagulative activity of earthworm extract by APTT test. The study involves the extraction and isolation of anticoagulant from earthworm which was found to be in the form of DNA. In order to uncover the biochemical nature of this molecule, the anticoagulant was processed with various hydrolases such as Proteinase-K, Dnase, Rnase and lysozyme. Simultaneously APTT test and agarose gel electrophoresis were performed to confirm the results. Standard herring sperm DNA and yeast RNA were also used to compare the anticoagulative activities with that of anticoagulant purified from earthworm. Individual components of nucleotide were also checked which might be responsible for the anticoagulative capability.
ISSN:0972-3099
2278-5124
DOI:10.36953/ECJ.2011.121214