MALDI-TOF MS Identification and Clustering Applied to Enterobacter Species in Nosocomial Setting

microorganisms cause important bacterial infections in humans. Recently, carbapenem resistant isolates carrying the KPC gene were described and their clonal transmission in different nosocomial outbreaks reported. In this study, the relative numbers of species, their antimicrobial susceptibility alo...

Full description

Saved in:
Bibliographic Details
Published in:Frontiers in microbiology 2018-08, Vol.9, p.1885-1885
Main Authors: De Florio, Lucia, Riva, Elisabetta, Giona, Annalisa, Dedej, Etleva, Fogolari, Marta, Cella, Eleonora, Spoto, Silvia, Lai, Alessia, Zehender, Gianguglielmo, Ciccozzi, Massimo, Angeletti, Silvia
Format: Article
Language:English
Subjects:
clustering analysis
Enterobacter
MALDI-TOF mass spectrometry
Microbiology
nosocomial infection
resistance
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:microorganisms cause important bacterial infections in humans. Recently, carbapenem resistant isolates carrying the KPC gene were described and their clonal transmission in different nosocomial outbreaks reported. In this study, the relative numbers of species, their antimicrobial susceptibility along 3 years of observation and the identification ability of the two most common MALDI-TOF platforms were evaluated. A clustering analysis was performed to identify changes in the microbial population within the nosocomial environment. were identified using two platforms (MALDI-TOF Biotyper and VITEK MS). Antimicrobial susceptibility was tested by Vitek2 Compact and MIC and MIC was evaluated using GraphPad software. Clustering analysis was performed by MALDI-TOF and a dendrogram was built with both platforms and compared. The most frequent species isolated were and with a gradual increase of in 2017. MALDI-TOF platforms showed a very good sensitivity and specificity except for identification that was reliable only by MALDI-TOF MS Biotyper. An increase of resistance for , confirmed by the isolation of extended spectrum beta-lactamase (ESBL) strains and the emergence of multidrug-resistant (MDR) and carbapenem resistant strains, was observed. A clonal route of transmission involving general surgery and geriatric wards was evidenced as previously described for MDR strains in the same nosocomial setting. These data represent an important source of information about the spreading of in the nosocomial environment.
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2018.01885