Geniposide suppressed OX-LDL-induced osteoblast apoptosis by regulating the NRF2/NF-κB signaling pathway

BackgroundOsteoporosis (OP), due to microarchitectural alterations, is associated with decreased bone mass, declined strength, and increased fracture risk. Increased osteoblast apoptosis contributes to the progression of OP. Natural compounds from herbs provide a rich resource for drug screening. Ou...

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Published in:Journal of orthopaedic surgery and research 2023-08, Vol.18 (1), p.1-641, Article 641
Main Authors: Xiao, Yaosheng, Zhang, Shanshan, Ye, Yongjun, Chen, Jincai, Xu, Youjia
Format: Article
Language:English
Subjects:
Animal models
Antibodies
Apoptosis
Bone mass
Cholesterol
Diabetes
Drug screening
Dual energy X-ray absorptiometry
Ethanol
Geniposide
High density lipoprotein
High fat diet
Hyperlipidemia
Immunohistochemistry
Laboratory animals
Low density lipoprotein
NF-κB protein
NRF2/NF-κB
Orthopedics
Osteoblast
Osteoblasts
Osteoporosis
Proteins
Signal transduction
Statistical analysis
Variance analysis
Western blotting
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Summary:BackgroundOsteoporosis (OP), due to microarchitectural alterations, is associated with decreased bone mass, declined strength, and increased fracture risk. Increased osteoblast apoptosis contributes to the progression of OP. Natural compounds from herbs provide a rich resource for drug screening. Our previous investigation showed that geniposide (GEN), an effective compound from Eucommia ulmoides, could protect against the pathological development of OP induced by cholesterol accumulation.MethodsThe rat OP models were duplicated. Dual-energy X-ray absorptiometry, hematoxylin and eosin staining, and immunohistochemistry were used to evaluate bone changes. TUNEL/DAPI staining assays were used for cell apoptosis detection. Protein expression was determined by western blotting assays.ResultsA high-fat diet promoted OP development in vivo, and OX-LDL stimulated osteoblast apoptosis in vitro. GEN exhibited protective activities against OX-LDL-induced osteoblast apoptosis by increasing the NRF2 pathway and decreasing the NF-κB pathway. PDTC, an NF-κB inhibitor, could further promote the biological functions of GEN. In contrast, ML385, an NRF2 inhibitor, might eliminate GEN’s protection.ConclusionGEN suppressed OX-LDL-induced osteoblast apoptosis by regulating the NRF2/NF-κB signaling pathway.
ISSN:1749-799X
1749-799X
DOI:10.1186/s13018-023-04125-5