Methicillin-Resistant Staphylococcus aureus from Peninsular Malaysian Animal Handlers: Molecular Profile, Antimicrobial Resistance, Immune Evasion Cluster and Genotypic Categorization

( ) infections, particularly methicillin-resistant (MRSA) in humans and animals, have become a significant concern globally. The present study aimed to determine the prevalence and antibiogram of isolated from animal handlers in Peninsular Malaysia. Furthermore, the genotypic characteristics of isol...

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Bibliographic Details
Published in:Antibiotics (Basel) 2022-01, Vol.11 (1), p.103
Main Authors: Chai, Minhian, Sukiman, Muhammad Zikree, Kamarun Baharin, Amirah Huda, Ramlan, Insyirah, Lai, Lennard Zhunhoong, Liew, Yeewen, Malayandy, Pavitra, Mohamad, Noor Muzamil, Choong, Siewshean, Ariffin, Siti Mariam Zainal, Ghazali, Mohd Faizal
Format: Article
Language:English
Subjects:
Amoxicillin
Animals
antibiotic resistance genes
Antibiotics
Antimicrobial agents
Antimicrobial resistance
Bacteria
Clusters
Drug resistance
Epidemiology
Erythromycin
Genes
Genetic diversity
Genotypes
Gentamicin
Handlers
Hogs
Immune evasion
Linezolid
Livestock
Methicillin
Penicillin
Pets
Poultry
spa typing
Spas
Staphylococcus aureus
Staphylococcus infections
Surveillance
Veterinarians
Virulence
virulence genes
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Summary:( ) infections, particularly methicillin-resistant (MRSA) in humans and animals, have become a significant concern globally. The present study aimed to determine the prevalence and antibiogram of isolated from animal handlers in Peninsular Malaysia. Furthermore, the genotypic characteristics of isolates were also investigated. Nasal and oral swab samples were collected from 423 animal handlers in Peninsular Malaysia. The antibiogram profiles of against 18 antibiotics were established using a Kirby-Bauer test. The genotypic profile of , including the presence of antimicrobial resistance (AMR), virulence genes and genotypes, was investigated using molecular techniques. The overall carriage rate of , MRSA and MDRSA was 30.5%, 1.2% and 19.4%, respectively. was highly resistant against penicillin (72.3%) and amoxicillin (52.3%). Meanwhile, gentamicin and linezolid were fully effective against all the isolated from animal handlers. It was observed that animal handlers with close exposure to poultry were more likely to carry that is resistant to tetracycline and erythromycin. isolates harboured tetracycline resistance ( K, L and M), erythromycin resistance ( , , and ) and immune evasion cluster (IEC) genes ( , , and ). Seventeen different types were detected among the 30 isolates of MDRSA, with t189 (16.7%) and t4171 (16.7%) being the predominant type, suggesting wide genetic diversity of the MDRSA isolates. The present study demonstrated the prevalence of strains, including MRSA and MDRSA with various antimicrobial resistance and genetic profiles from animal handlers in Peninsular Malaysia.
ISSN:2079-6382
2079-6382
DOI:10.3390/antibiotics11010103