PeGSTU58, a Glutathione S-Transferase from Populus euphratica , Enhances Salt and Drought Stress Tolerance in Transgenic Arabidopsis

Glutathione S-transferases (GSTs) play a crucial role in responding to abiotic stress and are an important target for research on plant stress tolerance mechanisms. is a promising candidate species for investigating the abiotic tolerance mechanisms in woody plants. In our previous study, was identif...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2023-05, Vol.24 (11), p.9354
Main Authors: Meng, Huijing, Zhao, Jinna, Yang, Yanfei, Diao, Kehao, Zheng, Guangshun, Li, Tao, Dai, Xinren, Li, Jianbo
Format: Article
Language:English
Subjects:
Abiotic stress
Arabidopsis
Cytoplasm
Deserts
Drought
drought stress
Flowers & plants
Genes
Glutathione transferase
Homeostasis
Localization
Oxidative stress
PeGSTU58
Plant stress
Populus
Populus euphratica
Proteins
Salinity
Salinity tolerance
salt stress
Superoxide dismutase
Transcription factors
Transgenic plants
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Glutathione S-transferases (GSTs) play a crucial role in responding to abiotic stress and are an important target for research on plant stress tolerance mechanisms. is a promising candidate species for investigating the abiotic tolerance mechanisms in woody plants. In our previous study, was identified as being associated with seed salinity tolerance. In the present study, was cloned from . and functionally characterized. encodes a Tau class GST and is located in both the cytoplasm and nucleus. Transgenic overexpressing displayed enhanced tolerance to salt and drought stress. Under salt and drought stress, the transgenic plants exhibited significantly higher activities of antioxidant enzymes, including SOD, POD, CAT, and GST, compared to the wild-type (WT) plants. Additionally, the expression levels of several stress-responsive genes, including , , , , and were upregulated in overexpression lines compared to those in WT under salt and drought stress conditions. Furthermore, yeast one-hybrid assays and luciferase analysis showed that PebHLH35 can directly bind to the promoter region of and activate its expression. These results indicated that was involved in salt and drought stress tolerances by maintaining ROS homeostasis, and its expression was positively regulated by PebHLH35.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms24119354