IgE re-programs alternatively-activated human macrophages towards pro-inflammatory anti-tumoural statesResearch in context

Background: Antibody Fc-driven engagement of macrophages is critical for evoking cellular activation and effector functions and influencing tumour-associated macrophage (TAM) recruitment. We previously reported that IgE class antibodies promote restriction of cancer growth in rodent models associate...

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Bibliographic Details
Published in:EBioMedicine 2019-05, Vol.43, p.67-81
Main Authors: Giulia Pellizzari, Coran Hoskin, Silvia Crescioli, Silvia Mele, Jelena Gotovina, Giulia Chiaruttini, Rodolfo Bianchini, Kristina Ilieva, Heather J. Bax, Sophie Papa, Katie E. Lacy, Erika Jensen-Jarolim, Sophia Tsoka, Debra H. Josephs, James F. Spicer, Sophia N. Karagiannis
Format: Article
Language:English
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Summary:Background: Antibody Fc-driven engagement of macrophages is critical for evoking cellular activation and effector functions and influencing tumour-associated macrophage (TAM) recruitment. We previously reported that IgE class antibodies promote restriction of cancer growth in rodent models associated with significant TAM infiltration. However, the human macrophage-associated IgE-Fc Receptor (FcεR) axis remains unexplored. We investigated the effects of anti-tumour IgE stimulation on human macrophage activation. Methods: Human blood monocyte-differentiated quiescent (M0), classically-(M1) and alternatively-(M2) activated macrophages were crosslinked with IgE and polyclonal antibodies to mimic immune complex formation. We examined surface marker expression, cytokine secretion, protein kinase phosphorylation and gene expression in IgE-stimulated macrophages and IgE antibody-dependent macrophage-mediated cytotoxicity (ADCC) against tumour cells. Findings: A proportion (40%) of M2 and (
ISSN:2352-3964
2352-3964