Core oligosaccharide of Plesiomonas shigelloides PCM 2231 (Serotype O17) lipopolysaccharide--structural and serological analysis

The herein presented complete structure of the core oligosaccharide of lipopolysaccharide (LPS) P. shigelloides Polish Collection of Microorganisms (PCM) 2231 (serotype O17) was investigated by (1)H, (13)C NMR spectroscopy, mass spectrometry, chemical analyses and serological methods. The core oligo...

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Bibliographic Details
Published in:Marine drugs 2013-02, Vol.11 (2), p.440-454
Main Authors: Maciejewska, Anna, Lukasiewicz, Jolanta, Kaszowska, Marta, Man-Kupisinska, Aleksandra, Jachymek, Wojciech, Lugowski, Czeslaw
Format: Article
Language:English
Subjects:
Animals
Carbohydrate Conformation
core oligosaccharide
endotoxin
lipopolysaccharide
Lipopolysaccharides - chemistry
Lipopolysaccharides - metabolism
Magnetic Resonance Spectroscopy
Marine
Mass Spectrometry
Oligosaccharides - chemistry
Oligosaccharides - metabolism
Plesiomonas - classification
Plesiomonas - metabolism
Plesiomonas shigelloides
Rabbits
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Summary:The herein presented complete structure of the core oligosaccharide of lipopolysaccharide (LPS) P. shigelloides Polish Collection of Microorganisms (PCM) 2231 (serotype O17) was investigated by (1)H, (13)C NMR spectroscopy, mass spectrometry, chemical analyses and serological methods. The core oligosaccharide is composed of an undecasaccharide, which represents the second core type identified for P. shigelloides serotype O17 LPS. This structure is similar to that of the core oligosaccharide of P. shigelloides strains 302-73 (serotype O1) and 7-63 (serotype O17) and differs from these only by one sugar residue. Serological screening of 55 strains of P. shigelloides with the use of serum against identified core oligosaccharide conjugated with bovine serum albumin (BSA) indicated the presence of similar structures in the LPS core region of 28 O-serotypes. This observation suggests that the core oligosaccharide structure present in strain PCM 2231 could be the most common type among P. shigelloides lipopolysaccharides.
ISSN:1660-3397
1660-3397
DOI:10.3390/md11020440