Complex-Forming Properties of the Anti-Inflammatory Sialorphin Derivative Palmitic Acid-Lysine-Lysine-Glutamine-Histidine-Asparagine-Proline-Arginine with Cu(II) Ions in an Aqueous Solution

• Published in: Molecules (Basel, Switzerland) Switzerland), 2023-12, Vol.29 (1), p.90
• Main Authors: Pająk, Marek, Kamysz, Elżbieta, Sikora, Karol, Fichna, Jakub, Woźniczka, Magdalena
• Format: Article
• Language: English
• Subjects: Amino acids; Anti-inflammatory drugs; Aqueous solutions; Cholesterol; Cu(II) complexes; Edema; Enkephalins; Fatty acids; High density lipoprotein; Inflammation; Ligands; Narcotics; Natriuretic peptides; palmitic acid; Peptides; Physiology; potentiometry; protonation constant; Saturated fatty acids; sialorphin; Spectrum analysis; Triglycerides; UV-Vis spectroscopy; Vegetable oils
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules29010090

The present work describes the complexation of the anti-inflammatory sialorphin derivative Pal-Lys-Lys-Gln-His-Asn-Pro-Arg (palmitic acid-lysine-lysine-glutamine-histidine-asparagine-proline-arginine) with Cu(II) ions in an aqueous solution, at a temperature of 25.0 ± 0.1 °C, over the whole pH range. The complexing properties were characterized by potentiometric and UV-Vis spectrophotometric methods. The potentiometric method was used to calculate the logarithms of the overall stability constants (log ) and the values of the stepwise dissociation constants (p ) of the studied complexes. The percentage of each species formed in an aqueous solution was estimated from the species distribution curve as a function of pH. The absorbance ( ) and molar absorption coefficient ( ) values for the Cu(II)-sialorphin derivative system were determined with UV-Vis spectroscopy. Our studies indicate that the sialorphin derivative forms stable complexes with Cu(II) ions, which may lead to future biological and therapeutic applications.