Detection of Staphylococcus epidermidis by a Quartz Crystal Microbalance Nucleic Acid Biosensor Array Using Au Nanoparticle Signal Amplification

Staphylococcus epidermidis is a critical pathogen of nosocomial blood infections, resulting in significant morbidity and mortality. A piezoelectric quartz crystal microbalance (QCM) nucleic acid biosensor array using Au nanoparticle signal amplification was developed to rapidly detect S. epidermidis...

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Bibliographic Details
Published in:Sensors (Basel, Switzerland) Switzerland), 2008-10, Vol.8 (10), p.6453-6470
Main Authors: Xia, Han, Wang, Feng, Huang, Qing, Huang, Junfu, Chen, Ming, Wang, Jue, Yao, Chunyan, Chen, Qinghai, Cai, Guoru, Fu, Weiling
Format: Article
Language:English
Subjects:
Acids
Au nanoparticle
biosensor
Biosensors
Hybridization
Infections
Ligands
Nanoparticles
quartz crystal microbalance
RecA protein
S. epidermidis
Sensors
Virulence
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Summary:Staphylococcus epidermidis is a critical pathogen of nosocomial blood infections, resulting in significant morbidity and mortality. A piezoelectric quartz crystal microbalance (QCM) nucleic acid biosensor array using Au nanoparticle signal amplification was developed to rapidly detect S. epidermidis in clinical samples. The synthesized thiolated probes specific targeting S. epidermidis 16S rRNA gene were immobilized on the surface of QCM nucleic acid biosensor arrays. Hybridization was induced by exposing the immobilized probes to the PCR amplified fragments of S. epidermidis, resulting in a mass change and a consequent frequency shift of the QCM biosensor. To further enhance frequency shift results from above described hybridizations, streptavidin coated Au nanoparticles were conjugated to the PCR amplified fragments. The results showed that the lowest detection limit of current QCM system was 1.3×10³ CFU/mL. A linear correlation was found when the concentration of S. epidermidis varied from 1.3×10³ to 1.3×10 CFU/mL. In addition, 55 clinical samples were detected with both current QCM biosensor system and conventional clinical microbiological method, and the sensitivity and specificity of current QCM biosensor system were 97.14% and 100%, respectively. In conclusion, the current QCM system is a rapid, low-cost and sensitive method that can be used to identify infection of S. epidermidis in clinical samples.
ISSN:1424-8220
1424-8220
DOI:10.3390/s8106453