Characterization of Perionyx excavatus Development and Its Head Regeneration

Regeneration is a biological process restoring lost or amputated body parts. The capability of regeneration varies among organisms and the regeneration of the central nervous system (CNS) is limited to specific animals, including the earthworm Perionyx excavatus. Thus, it is crucial to establish P....

Full description

Saved in:
Bibliographic Details
Published in:Biology (Basel, Switzerland) Switzerland), 2020-09, Vol.9 (9), p.273
Main Authors: Bae, Yun Seon, Kim, Jung, Yi, Jeesoo, Park, Soon Cheol, Lee, Hae-Youn, Cho, Sung-Jin
Format: Article
Language:English
Subjects:
Biological research
Cell culture
Cell differentiation
Cell growth
Cell proliferation
Central nervous system
Cytology
earthworm
Earthworms
embryonic development
Embryos
Head
head regeneration
juvenile
Juveniles
Laboratories
Life cycles
Morphogenesis
Myogenesis
Neurogenesis
Perionyx excavatus
Physiological aspects
Regeneration
Regeneration (Biology)
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Regeneration is a biological process restoring lost or amputated body parts. The capability of regeneration varies among organisms and the regeneration of the central nervous system (CNS) is limited to specific animals, including the earthworm Perionyx excavatus. Thus, it is crucial to establish P. excavatus as a model system to investigate mechanisms of CNS regeneration. Here, we set up a culture system to sustain the life cycle of P. excavatus and characterize the development of P. excavatus, from embryo to juvenile, based on its morphology, myogenesis and neurogenesis. During development, embryos have EdU-positive proliferating cells throughout the whole body, whereas juveniles maintain proliferating cells exclusively in the head and tail regions, not in the trunk region. Interestingly, juveniles amputated at the trunk, which lacks proliferating cells, are able to regenerate the entire head. In this process, a group of cells, which are fully differentiated, reactivates cell proliferation. Our data suggest that P. excavatus is a model system to study CNS regeneration, which is dependent on the dedifferentiation of cells.
ISSN:2079-7737
2079-7737
DOI:10.3390/biology9090273