Hypoxia-Responsive Class III Peroxidases in Maize Roots: Soluble and Membrane-Bound Isoenzymes

• Published in: International journal of molecular sciences 2020-11, Vol.21 (22), p.8872
• Main Authors: Hofmann, Anne, Wienkoop, Stefanie, Harder, Sönke, Bartlog, Fabian, Lüthje, Sabine
• Format: Article
• Language: English
• Subjects: Abiotic stress; aerenchyma; Anoxia; Apoptosis; Cell Hypoxia - genetics; Cell Membrane - genetics; Cell Wall - genetics; cell wall remodeling; Cell walls; Cellulose; Chromatography, Liquid; class III peroxidases; Enzymes; Flooding; Flowers & plants; Gene expression; Gene Expression Regulation, Plant; Guaiacol; Homology; Hydrogen peroxide; Hypoxia; Isoenzymes; Isoenzymes - genetics; Lignin; maize roots; Mass spectrometry; Mass spectroscopy; Membranes; NADPH Oxidases - genetics; Oxidation-Reduction; Peroxidase; Peroxidase - genetics; Peroxidases - genetics; Plant Roots - enzymology; Plant Roots - genetics; plasma membrane; Protein Binding - genetics; Proteins; Proteome - genetics; Proteomes; Reactive oxygen species; Reactive Oxygen Species - metabolism; Respiratory burst oxidase; Roots; Scavenging; Tandem Mass Spectrometry; Two dimensional analysis; Zea mays; Zea mays - enzymology; Zea mays - genetics
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21228872

Flooding induces low-oxygen environments (hypoxia or anoxia) that lead to energy disruption and an imbalance of reactive oxygen species (ROS) production and scavenging enzymes in plants. The influence of hypoxia on roots of hydroponically grown maize ( L.) plants was investigated. Gene expression (RNA Seq and RT-qPCR) and proteome (LC-MS/MS and 2D-PAGE) analyses were used to determine the alterations in soluble and membrane-bound class III peroxidases under hypoxia. Gel-free peroxidase analyses of plasma membrane-bound proteins showed an increased abundance of Prx03, Prx24, Prx81, and Pr85 in stressed samples. Furthermore, RT-qPCR analyses of the corresponding peroxidase genes revealed an increased expression. These peroxidases could be separated with 2D-PAGE and identified by mass spectrometry. An increased abundance of Prx03 and Prx85 was determined. Further peroxidases were identified in detergent-insoluble membranes. Co-regulation with a respiratory burst oxidase homolog (Rboh) and key enzymes of the phenylpropanoid pathway indicates a function of the peroxidases in membrane protection, aerenchyma formation, and cell wall remodeling under hypoxia. This hypothesis was supported by the following: (i) an elevated level of hydrogen peroxide and aerenchyma formation; (ii) an increased guaiacol peroxidase activity in membrane fractions of stressed samples, whereas a decrease was observed in soluble fractions; and (iii) alterations in lignified cells, cellulose, and suberin in root cross-sections.