CLCA1 suppresses colorectal cancer aggressiveness via inhibition of the Wnt/beta-catenin signaling pathway

Chloride channel accessory 1 (CLCA1) belongs to the calcium-sensitive chloride conductance protein family, which is mainly expressed in the colon, small intestine and appendix. This study was conducted to investigate the functions and mechanisms of CLCA1 in colorectal cancer (CRC). The CLCA1 protein...

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Bibliographic Details
Published in:Cell communication and signaling 2017-10, Vol.15 (1), p.38-38, Article 38
Main Authors: Li, Xiaofen, Hu, Wangxiong, Zhou, Jiaojiao, Huang, Yanqin, Peng, Jiaping, Yuan, Ying, Yu, Jiekai, Zheng, Shu
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Breast cancer
Calcium chloride
Calcium conductance
Care and treatment
Cell cycle
Cell Line, Tumor
Cell Movement
Cell Proliferation
Cell Transformation, Neoplastic
Chloride
Chloride channel accessory 1
Chloride channels (calcium-gated)
Chloride Channels - metabolism
Chloride conductance
Colorectal cancer
Colorectal carcinoma
Colorectal Neoplasms - metabolism
Colorectal Neoplasms - pathology
CRISPR
Diagnosis
Early detection
Enzyme-linked immunosorbent assay
Enzymes
Epithelial cells
Epithelial-Mesenchymal Transition
Female
Gene expression
Humans
Immunohistochemistry
Kinases
Male
Medical prognosis
Mesenchyme
Metastases
Metastasis
Mice
Patients
Proteins
Rodents
Signal transduction
Small intestine
Studies
Tumor suppressor
Tumor suppressor genes
Western blotting
Wnt protein
Wnt Signaling Pathway
β-Catenin
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Description
Summary:Chloride channel accessory 1 (CLCA1) belongs to the calcium-sensitive chloride conductance protein family, which is mainly expressed in the colon, small intestine and appendix. This study was conducted to investigate the functions and mechanisms of CLCA1 in colorectal cancer (CRC). The CLCA1 protein expression level in CRC patients was evaluated by enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC), and western blotting analysis. Using CRISPR/Cas9 technology, CLCA1-upregulated (CLCA1-ACT) and CLCA1-knockout cells (CLCA1-KO), as well as their respective negative controls (CLCA1-ACT-NC and CLCA1-KO-NC), were constructed from the SW620 cell line. Cell growth and metastatic ability were assessed both in vitro and in vivo. The association of CLCA1 with epithelial-mesenchymal transition (EMT) and other signaling pathways was determined by western blotting assays. The expression level of CLCA1 in CRC tissues was significantly decreased compared with that in adjacent normal tissue (P
ISSN:1478-811X
1478-811X
DOI:10.1186/s12964-017-0192-z