Genomic and Pathological Characterization of Acute Hepatopancreatic Necrosis Disease (AHPND)-Associated Natural Mutant Vibrio parahaemolyticus Isolated from Penaeus vannamei Cultured in Korea

Acute hepatopancreatic necrosis disease (AHPND) is one of the most important diseases in the global shrimp industry. The emergence of mutant AHPND-associated . ( ) strains has raised concerns regarding potential misdiagnosis and unforeseen pathogenicity. In this study, we report the first emergence...

Full description

Saved in:
Bibliographic Details
Published in:Animals (Basel) 2024-09, Vol.14 (19), p.2788
Main Authors: Kim, Ye Bin, Park, Seon Young, Jeon, Hye Jin, Kim, Bumkeun, Kwon, Mun-Gyeong, Kim, Su-Mi, Han, Jee Eun, Kim, Ji Hyung
Format: Article
Language:English
Subjects:
Antimicrobial agents
Aquaculture
Disease
Diseases
Genes
Genomes
Health aspects
Mortality
Necrosis
O1: non-typeable K
Pathogens
Plasmids
shrimp bioassay
South Korea
ST 413
strain 19-021-D1
Sulbactam
Tetracycline
Tetracyclines
Toxins
VpAHPND
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Acute hepatopancreatic necrosis disease (AHPND) is one of the most important diseases in the global shrimp industry. The emergence of mutant AHPND-associated . ( ) strains has raised concerns regarding potential misdiagnosis and unforeseen pathogenicity. In this study, we report the first emergence of a type II ( , ) natural mutant, (strain 20-082A3), isolated from cultured in Korea. Phenotypic and genetic analyses revealed a close relationship between the mutant strain 20-082A3 and the virulent Korean strain 19-021-D1, which caused an outbreak in 2019. Detailed sequence analysis of AHPND-associated plasmids showed that plasmid pVp_20-082A3B in strain 20-082A3 was almost identical (>99.9%) to that of strain 19-021-D1. Moreover, strains 20-082A3 and 19-021-D1 exhibited the same multilocus sequence type (ST 413) and serotype (O1:Un-typeable K-serogroup), suggesting that the mutant strain is closely related to and may have originated from the virulent strain 19-021-D1. Similar to previous reports on the natural mutant , strain 20-082A3 did not induce AHPND-related symptoms or cause mortality in the shrimp bioassay. The emergence of a mutant strain which is almost identical to the virulent highlights the need for surveillance of the pathogen prevalent in Korea. Further investigations to elucidate the potential relationship between ST 413 and recent Korean isolates are needed.
ISSN:2076-2615
2076-2615
DOI:10.3390/ani14192788