The First Swedish Outbreak with VIM-2-Producing Pseudomonas aeruginosa , Occurring between 2006 and 2007, Was Probably Due to Contaminated Hospital Sinks

• Published in: Microorganisms (Basel) 2023-04, Vol.11 (4), p.974
• Main Authors: Fraenkel, Carl-Johan, Starlander, Gustaf, Tano, Eva, Sütterlin, Susanne, Melhus, Åsa
• Format: Article
• Language: English
• Subjects: Antibiotics; Bacteria; Basic Medicine; Catheters; Clinical Medicine; Colistin; Control; Deoxyribonucleic acid; Distribution; DNA; Electrophoresis; Epidemics; Gene sequencing; Genes; Genetic aspects; Genomes; Gentamicin; Hospitals; Hygiene; Infectious diseases; Infectious Medicine; Infektionsmedicin; Klinisk medicin; Laboratories; MBL; Medical and Health Sciences; Medical equipment; Medicin och hälsovetenskap; Medicinska och farmaceutiska grundvetenskaper; Metallography; Microbiology in the medical area; Mikrobiologi inom det medicinska området; Multidrug resistance; Nosocomial infections; nosocomial outbreak; Outbreaks; Patients; Physiological aspects; Plasmids; Pseudomonas aeruginosa; Pulsed-field gel electrophoresis; sink; Sinkholes; Sinks; Surgery; Sweden; Testing; VIM-2; Whole genome sequencing; β Lactamase
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms11040974

Multidrug-resistant is an increasing clinical problem worldwide. The aim of this study was to describe the first outbreak of a Verona integron-borne metallo-ß-lactamase (VIM)-2-producing strain in Sweden and its expansion in the region. A cluster of multidrug-resistant appeared at two neighbouring hospitals in 2006. The isolates were characterized by PCR, pulsed-field gel electrophoresis (PFGE), and whole-genome sequencing. Patient charts, laboratory records, and hygiene routines were reviewed, and patients, staff, and the environment were screened. The investigation revealed a clonal outbreak of a VIM-2-producing strain belonging to the high-risk clonal complex 111, susceptible only to gentamicin and colistin. No direct contact between patients could be established, but most of them had stayed in certain rooms/wards weeks to months apart. Cultures from two sinks yielded growth of the same strain. The outbreak ended when control measures against the sinks were taken, but new cases occurred in a tertiary care hospital in the region. In conclusion, when facing prolonged outbreaks with this bacterium, sinks and other water sources in the hospital environment should be considered. By implementing proactive control measures to limit the bacterial load in sinks, the waterborne transmission of may be reduced.