Effect of Porphyromonas gingivalis Infection on Healing of Skeletal Muscle Injury: An In Vivo Study

infection has been associated with various systemic diseases and may cause delayed healing of muscle injury. However, the relationship between muscle injury healing and infection remains unclear. Our hypothesis was that infection delays the healing of muscle injuries. Fifty-six 8-week-old male Wista...

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Bibliographic Details
Published in:Dentistry journal 2024-10, Vol.12 (11), p.346
Main Authors: Shimizu, Shintaro, Hayashi, Kairi, Takeuchi, Yasuo, Tanabe, Gen, Churei, Hiroshi, Kobayashi, Hiroaki, Ueno, Toshiaki, Fueki, Kenji
Format: Article
Language:English
Subjects:
Animals
Bacteria
Calcaneus
Fibrosis
Gum disease
Healing
Health aspects
Immunohistochemistry
In vivo methods and tests
Infection
Infections
Injuries
Injury analysis
Monoclonal antibodies
motion analysis
Muscles
Musculoskeletal system
Myoblasts
Myocytes
MyoD protein
P. gingivalis
periodontitis
Porphyromonas gingivalis
Proteins
satellite cell
Skeletal muscle
skeletal muscle injury
Staining
Wound healing
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Summary:infection has been associated with various systemic diseases and may cause delayed healing of muscle injury. However, the relationship between muscle injury healing and infection remains unclear. Our hypothesis was that infection delays the healing of muscle injuries. Fifty-six 8-week-old male Wistar rats were randomly divided into two groups: sonicated was intraperitoneally administered in one group (PG group), whereas saline was administered in the other group (CO group). Skeletal muscle injury was induced via cardiotoxin injections in all animals. The cross-sectional area of regenerating muscle cells was evaluated by haematoxylin-eosin staining, and the degree of muscle fibrosis was evaluated by Masson's trichrome staining. The expression of paired box protein (Pax7) and myoblast determination protein (MyoD) and the identified stages of myocyte regeneration were analysed by immunohistochemical staining. Motion analysis was performed during walking. The cross-sectional area of muscle cells was significantly smaller in the PG group on days 7 and 14 post-injury than in the CO group. The Pax7+/MyoD- ratio was significantly lower in the PG group on day 1 post-injury than in the CO group. Motion analysis of treadmill walking showed that the PG group had a lower minimum calcaneal height on days 3 and 7 post-injury than the CO group. This study suggests that administration of sonicated in rats can delay the healing process of muscle injury. Further research is needed to understand this mechanism of delay of .
ISSN:2304-6767
2304-6767
DOI:10.3390/dj12110346