OCLN as a novel biomarker for prognosis and immune infiltrates in kidney renal clear cell carcinoma: an integrative computational and experimental characterization

• Published in: Frontiers in immunology 2023-09, Vol.14, p.1224904-1224904
• Main Authors: Jia, Zongming, Kong, Ying, Wang, Chengyu, Fu, Zhenyu, Tian, Zhen, Sun, Yizhang, Lin, Yuxin, Huang, Yuhua
• Format: Article
• Language: English
• Subjects: immune infiltration; Immunology; OCLN; prognostic biomarker; renal clear cell carcinoma; translational informatics
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2023.1224904

BackgroundOccludin (OCLN) is an important tight junction protein and has been reported to be abnormally expressed in the development of malignant tumors. However, its biomarker and carcinogenic roles in kidney renal clear cell carcinoma (KIRC) are less investigated.MethodsThe Cancer Genome Atlas database and Human Protein Atlas database were used to analyze the expression of OCLN in KIRC. UALCAN database and methylation-specific PCR assay were used to evaluate the methylation level of OCLN in KIRC. Univariate and multivariate Cox regression analyses were performed to model the prognostic significance of OCLN in KIRC patient cohorts. The correlation between OCLN expression and the immune cell infiltration, immune-related function and immune checkpoints were explored. Finally, EdU, scratch assay and transwell experiments were conducted to validate the role of OCLN in KIRC development.ResultsThe expression of OCLN was significantly downregulated in KIRC, compared with normal renal tissues (p