Morphological and molecular characterization of Stomachicola muraenesocis Yamaguti, 1934 (Digenea: Hemiuridae) from the daggertooth pike conger Muraenesox cinereus (Forsskål)

• Published in: Parasitology 2024-01, Vol.151 (1), p.24-44
• Main Authors: Ghanei-Motlagh, Reza, Hernández-Orts, Jesús S., Fast, Mark D., Whyte, Shona K., El-Matbouli, Mansour, Saleh, Mona
• Format: Article
• Language: English
• Subjects: 18S; 28S; Animals; Biogeography; Body size; Dinurinae; DNA, Ribosomal - genetics; Esocidae - genetics; Ethanol; Fish; Fishes; Hemiuridae; Hemiuroidea; Lecithasteridae; Microscopy; molecular characterization; Molecular Sequence Data; morphological variation; Morphology; Muraenesox cinereus; Phylogenetics; Phylogeny; rDNA sequence; Reproductive organs; RNA, Ribosomal, 28S - genetics; rRNA 28S; Scanning electron microscopy; Stomach; taxonomic revision; Taxonomy; Trematoda; trematode; Tubules; Vitellin
• ISSN: 0031-1820; 1469-8161; 1469-8161
• DOI: 10.1017/S0031182023001063

Hemiurid digeneans conspecific with Stomachicola muraenesocis Yamaguti, 1934 (the type species of the genus Stomachicola Yamaguti, 1934) were collected from the stomach of the daggertooth pike conger Muraenesox cinereus (Forsskål) off the Persian Gulf of Iran. This study aimed to provide a detailed characterization of Stom. muraenesocis, including measurements, illustrations and scanning electron microscopy (s.e.m.) representations. Comparisons with the original and previous descriptions revealed morphological and metrical variations in several features (i.e. body size and shape, arrangement of reproductive organs, soma to ecsoma length ratio, position of genital opening, number of vitelline tubules and extension of uterine coils) between Stom. muraenesocis from different hosts and localities. This study presents the first molecular sequence data associated with the small (18S) and large (28S) subunit nuclear ribosomal RNA genes (rDNA) for Stom. muraenesocis. Phylogenetic analyses of the 18S dataset placed Stom. muraenesocis as sister lineage to a clade formed of a group of species of Lecithaster Lühe, 1901 (Lecithasteridae Odhner, 1905). In contrast, phylogenetic analyses based on the 28S consistently recovered a sister relationship between Stom. muraenesocis and representatives of the Hemiuridae Looss, 1899. Further comprehensive phylogenetically based classification in light of morphology and taxonomic history of the Hemiuridae and Lecithasteridae is required to infer phylogenetic affinities and historical biogeography of Stomachicola. A comprehensive list of previously reported species of Stomachicola together with their associated hosts, localities and morphometric data is provided.