Functional and Probiotic Characterization of Newly Isolated Strains from Infant Feces and Breast Milk

Infant feces and breast milk are sources of lactic acid bacteria with interesting functional and technological properties. Therefore, we focused on the isolation and characterization of new potential probiotic strains from infant feces and breast milk. Nine strains were identified based on 16S rRNA...

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Bibliographic Details
Published in:Fermentation (Basel) 2023-11, Vol.9 (11), p.960
Main Authors: Hyrslova, Ivana, Drab, Vladimir, Cihlar, Jaromir, Krausova, Gabriela, Mrvikova, Iva, Kana, Antonin, Stetina, Jiri, Musilova, Sarka
Format: Article
Language:English
Subjects:
Acids
adherence
Antibiotic resistance
Antibiotics
Antimicrobial activity
Antimicrobial agents
Babies
Bacteria
Breast milk
Breastfeeding & lactation
Cell lines
Composition
Drug resistance
Enzymatic activity
Exopolysaccharides
Feces
Food industry
Foodborne pathogens
Gastrointestinal tract
Hydrophobicity
Infants
Kanamycin
Lactic acid bacteria
Lacticaseibacillus rhamnosus
Lactobacillaceae
Lactobacillus gasseri
Microorganisms
Milk
Pathogens
Probiotics
rRNA 16S
Strains (organisms)
Streptomycin
Vancomycin
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Summary:Infant feces and breast milk are sources of lactic acid bacteria with interesting functional and technological properties. Therefore, we focused on the isolation and characterization of new potential probiotic strains from infant feces and breast milk. Nine strains were identified based on 16S rRNA sequences of Lacticaseibacillus rhamnosus, Limosilactobacillus reuteri, and Lactobacillus gasseri. Subsequently, these isolates were evaluated for their safety (antibiotic resistance, hemolytic, and enzymatic activity) and functional properties (gastrointestinal condition tolerance, hydrophobicity, auto-aggregation, adhesion to Caco-2/HT-29 cell lines, antimicrobial and antioxidant properties, and the production of exopolysaccharides). All the isolates were non-hemolytic and resistant to streptomycin, kanamycin, and vancomycin. They also demonstrated antimicrobial activity against five selected pathogens (Staphylococcus aureus, Bacillus cereus, Escherichia coli, Salmonella sp., and Enterococcus faecalis) and the production of exopolysaccharides. Isolates Lacticaseibacillus rhamnosus S1F and S6C together with Lactobacillus gasseri M8C showed the highest ability to survive 3.0 g/L of bile salts and a good adhesion property, which is important for gastrointestinal tract (GIT) colonization or the inhibition of pathogens. Following in vivo characterization, three strains S1F and S6C from infant feces, and M6C from breast milk, showed probiotic properties with potential applications in the food industry.
ISSN:2311-5637
2311-5637
DOI:10.3390/fermentation9110960