Transcriptional regulation mechanism of wheat varieties with different nitrogen use efficiencies in response to nitrogen deficiency stress

• Published in: BMC genomics 2022-10, Vol.23 (1), p.1-727, Article 727
• Main Authors: Wang, Hanxia, Ma, Qiaoyun, Shan, Fuhua, Tian, Liping, Gong, Jie, Quan, Wei, Yang, Weibing, Hou, Qiling, Zhang, Fengting, Zhang, Shengquan
• Format: Article
• Language: English
• Subjects: Abscisic acid; Agricultural production; Agricultural research; Agrochemicals; Alanine; Biomarkers; Biosynthesis; Calvin cycle; Crop yield; Cultivars; Cultivation; Dehydrogenases; Efficiency; Enzymes; Fertilizers; Gene expression; Gene regulation; Genetic aspects; Genetic transcription; Genomics; Germplasm; Grain cultivation; Hardiness; Helix-loop-helix proteins (basic); Heterosis; Metabolic regulation; Nitrates; Nitrogen; Nitrogen metabolism; Nitrogen use efficiency; Nutrient content; Photorespiration; Physiological aspects; Physiology; Plant breeding; Plants; Potassium; Regulatory mechanism; Seeds; Signal transduction; Trace elements; Transcription factors; Transcriptome analysis; Transcriptomes; Vitamin B6; Wheat
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-022-08948-0

As one of the microelements, nitrogen play essential roles in cereal production. Although the use of chemical fertilizers has significantly improved the yield of wheat, it has also caused increasingly adverse environmental pollution. Revealing the molecular mechanism manipulating wheat nitrogen use efficiency (NUE), and cultivating wheat germplasms with high nitrogen use efficiency has become important goals for wheat researchers. In this study, we investigated the physiological and transcriptional differences of three wheat cultivars with different NUE under low nitrogen stress. The results showed that, under low nitrogen conditions, the activities of nitrogen metabolism-related enzymes (GS, NR, GDH), antioxidant enzymes (SOD, POD, CAT) and soluble protein contents of ZM366 (high NUE cultivar) were higher than those of JD8 (low NUE cultivar). The hybrid cultivar of ZM366 and JD8 showed mid-parent or over-parent heterosis. Transcriptome analysis revealed that 'alanine, aspartate and glutamate metabolism', 'terpenoid backbone biosynthesis' and 'vitamin B6 metabolism' pathways play key roles in nitrogen use efficiency in wheat. The significant enhancement of the 'Calvin cycle' and 'photorespiration' in ZM366 contributed to its higher level of carbon metabolism under low nitrogen stress, which is an important attribute differs from the other two varieties. In addition, the activation of ABA signal transduction and biosynthesis pathways also helps to maintain NUE under low- nitrogen conditions. Moreover, bHLH transcription factors were also found to play a positive role in wheat NUE. In conclusion, these results enriched our knowledge of the mechanism of wheat NUE, and provided a theoretical basis for improving wheat NUE and breeding new cultivars.