Differential Pathogenic Gene Expression of E. histolytica in Patients with Different Clinical Forms of Amoebiasis

The etiological agent of human amoebiasis is the protozoan parasite E. histolytica; the disease is still an endemic infection in some countries and the outcome of infection in the host infection can range from asymptomatic intestinal infection to intestinal or liver invasive forms of the disease. Th...

Full description

Saved in:
Bibliographic Details
Published in:Microorganisms (Basel) 2020-10, Vol.8 (10), p.1556
Main Authors: González-Rivas, Enrique, Nieves-Ramírez, Miriam, Magaña, Ulises, Morán, Patricia, Rojas-Velázquez, Liliana, Hernández, Eric, Serrano-Vázquez, Angélica, Partida, Oswaldo, Pérez-Juárez, Horacio, Ximénez, Cecilia
Format: Article
Language:English
Subjects:
Abscesses
amebic liver abscess
Antibodies
Antigens
Asymptomatic
Biopsy
Calreticulin
Cysts
differential expression
Entamoeba histolytica
Environmental conditions
Environmental effects
Etiology
Feces
Gene expression
Genes
genital amoebiasis
Heat shock proteins
Hsp70 protein
Infections
Inflammatory bowel disease
Intestine
Isoleucine
Laboratories
Liver
Medicine
Parasites
pathogenic genes
Pathogenicity
Pathogens
Patients
Peroxiredoxin
Protozoa
quantitative polymerase chain reaction
Serine
Superoxide dismutase
Threonine
Trophozoites
Ulcers
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The etiological agent of human amoebiasis is the protozoan parasite E. histolytica; the disease is still an endemic infection in some countries and the outcome of infection in the host infection can range from asymptomatic intestinal infection to intestinal or liver invasive forms of the disease. The invasive character of this parasite is multifactorial and mainly due to the differential expression of multiple pathogenic genes. The aim of the present work was to measure the differential expression of some genes in different specimens of patients with amoebic liver abscess (ALA) and specimens of genital amoebiasis (AG) by RT-qPCR. Results show that the expression of genes is different in both types of samples. Almost all studied genes were over expressed in both sets of patients; however, superoxide dismutase (Ehsod), serine threonine isoleucine rich protein (Ehstirp), peroxiredoxin (Ehprd) and heat shock protein 70 and 90 (Ehhsp-70, EHhsp-90) were higher in AG biopsies tissue. Furthermore, cysteine proteinases 5 and 2 (Ehcp5, Ehcp2), lectin (Ehgal/galnaclectin) and calreticulin (Ehcrt) genes directly associate with pathogenic mechanisms of E. histolytica had similar over expression in both AG and ALA samples. In summary the results obtained show that trophozoites can regulate the expression of their genes depending on stimuli or environmental conditions, in order to regulate their pathogenicity and ensure their survival in the host.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms8101556