Tissue Barrier-on-Chip: A Technology for Reproducible Practice in Drug Testing

One key application of organ-on-chip systems is the examination of drug transport and absorption through native cell barriers such the blood–brain barrier. To overcome previous hurdles related to the transferability of existing static cell cultivation protocols and polydimethylsiloxane (PDMS) as the...

Full description

Saved in:
Bibliographic Details
Published in:Pharmaceutics 2022-07, Vol.14 (7), p.1451
Main Authors: Koch, Eugen V., Ledwig, Verena, Bendas, Sebastian, Reichl, Stephan, Dietzel, Andreas
Format: Article
Language:English
Subjects:
Ablation
Brain research
cell assay
Cell culture
cell seeding
Electrodes
Flow velocity
Lasers
membrane
organ-on-chip
Pharmaceuticals
Physiology
Polymers
Reproducibility
resealing technique
Silicon nitride
tissue barrier
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:One key application of organ-on-chip systems is the examination of drug transport and absorption through native cell barriers such the blood–brain barrier. To overcome previous hurdles related to the transferability of existing static cell cultivation protocols and polydimethylsiloxane (PDMS) as the construction material, a chip platform with key innovations for practical use in drug-permeation testing is presented. First, the design allows for the transfer of barrier-forming tissue into the microfluidic system after cells have been seeded on porous polymer or Si3N4 membranes. From this, we can follow highly reproducible models and cultivation protocols established for static drug testing, from coating the membrane to seeding the cells and cell analysis. Second, the perfusion system is a microscopable glass chip with two fluid compartments with transparent embedded electrodes separated by the membrane. The reversible closure in a clamping adapter requires only a very thin PDMS sealing with negligible liquid contact, thereby eliminating well-known disadvantages of PDMS, such as its limited usability in the quantitative measurements of hydrophobic drug molecule concentrations. Equipped with tissue transfer capabilities, perfusion chamber inertness and air bubble trapping, and supplemented with automated fluid control, the presented system is a promising platform for studying established in vitro models of tissue barriers under reproducible microfluidic perfusion conditions.
ISSN:1999-4923
1999-4923
DOI:10.3390/pharmaceutics14071451