Detection and characterization of bacterial endosymbionts in Southeast Asian tephritid fruit fly populations

Various endosymbiotic bacteria, including Wolbachia of the Alphaproteobacteria, infect a wide range of insects and are capable of inducing reproductive abnormalities to their hosts such as cytoplasmic incompatibility (CI), parthenogenesis, feminization and male-killing. These extended phenotypes can...

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Bibliographic Details
Published in:BMC microbiology 2019-12, Vol.19 (Suppl 1), p.290-18, Article 290
Main Authors: Asimakis, Elias D, Doudoumis, Vangelis, Hadapad, Ashok B, Hire, Ramesh S, Batargias, Costas, Niu, Changying, Khan, Mahfuza, Bourtzis, Kostas, Tsiamis, George
Format: Article
Language:English
Subjects:
16S rRNA
Abnormalities
Agricultural pests
Alphaproteobacteria
Analysis
Arsenophonus
Bacteria
Bactrocera scutellaris
Bactrocera zonata
Candidatus Cardinium
Cardinium
Cell division
Chromosomes
Cytoplasmic incompatibility
Dacus
Endosymbionts
Entomoplasmatales
Females
Feminization
Fruit flies
Fruit flies (Tephritidae)
Fruits
Gene transfer
Genes
Genetic research
Genomes
Genomics
Genotypes
Horizontal transfer
Incompatibility
Infections
Insects
Males
Medical tests
Multi locus sequence typing
Natural populations
Nucleotides
Organic farming
Parasites
Parthenogenesis
Pest control
Pests
Phenotypes
Phylogenetics
Phylogeny
Populations
Proteins
Pseudogenes
RNA
rRNA 16S
Sequence analysis
Spiroplasma
Sterilized organisms
Strains (organisms)
Wolbachia
Zeugodacus
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Summary:Various endosymbiotic bacteria, including Wolbachia of the Alphaproteobacteria, infect a wide range of insects and are capable of inducing reproductive abnormalities to their hosts such as cytoplasmic incompatibility (CI), parthenogenesis, feminization and male-killing. These extended phenotypes can be potentially exploited in enhancing environmentally friendly methods, such as the sterile insect technique (SIT), for controlling natural populations of agricultural pests. The goal of the present study is to investigate the presence of Wolbachia, Spiroplasma, Arsenophonus and Cardinium among Bactrocera, Dacus and Zeugodacus flies of Southeast Asian populations, and to genotype any detected Wolbachia strains. A specific 16S rRNA PCR assay was used to investigate the presence of reproductive parasites in natural populations of nine different tephritid species originating from three Asian countries, Bangladesh, China and India. Wolbachia infections were identified in Bactrocera dorsalis, B. correcta, B. scutellaris and B. zonata, with 12.2-42.9% occurrence, Entomoplasmatales in B. dorsalis, B. correcta, B. scutellaris, B. zonata, Zeugodacus cucurbitae and Z. tau (0.8-14.3%) and Cardinium in B. dorsalis and Z. tau (0.9-5.8%), while none of the species tested, harbored infections with Arsenophonus. Infected populations showed a medium (between 10 and 90%) or low (
ISSN:1471-2180
1471-2180
DOI:10.1186/s12866-019-1653-x