Quantitative 3D analysis of complex single border cell behaviors in coordinated collective cell migration

Understanding the mechanisms of collective cell migration is crucial for cancer metastasis, wound healing and many developmental processes. Imaging a migrating cluster in vivo is feasible, but the quantification of individual cell behaviours remains challenging. We have developed an image analysis t...

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Bibliographic Details
Published in:Nature communications 2017-04, Vol.8 (1), p.14905-14905, Article 14905
Main Authors: Cliffe, Adam, Doupé, David P., Sung, HsinHo, Lim, Isaac Kok Hwee, Ong, Kok Haur, Cheng, Li, Yu, Weimiao
Format: Article
Language:English
Subjects:
14/63
631/1647/48
631/1647/794
631/80/84/2334
Animals
Behavior
Cell adhesion & migration
Cell Movement - physiology
Cell Tracking - methods
Drosophila
Female
Humanities and Social Sciences
Image Processing, Computer-Assisted
Imaging, Three-Dimensional - methods
Insects
Metastasis
Microscopy, Confocal
Morphology
multidisciplinary
Oocytes
Ovary - cytology
Rotation
Science
Science (multidisciplinary)
Wound healing
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Summary:Understanding the mechanisms of collective cell migration is crucial for cancer metastasis, wound healing and many developmental processes. Imaging a migrating cluster in vivo is feasible, but the quantification of individual cell behaviours remains challenging. We have developed an image analysis toolkit, CCMToolKit, to quantify the Drosophila border cell system. In addition to chaotic motion, previous studies reported that the migrating cells are able to migrate in a highly coordinated pattern. We quantify the rotating and running migration modes in 3D while also observing a range of intermediate behaviours. Running mode is driven by cluster external protrusions. Rotating mode is associated with cluster internal cell extensions that could not be easily characterized. Although the cluster moves slower while rotating, individual cells retain their mobility and are in fact slightly more active than in running mode. We also show that individual cells may exchange positions during migration. Quantifying cell behaviours in vivo is essential to understanding the mechanisms of collective cell migration. Here the authors present an image analysis toolkit, CCMToolKit, to describe and characterize various modes of coordinated cell movements accompanying collective cell migration in Drosophila border cells.
ISSN:2041-1723
2041-1723
DOI:10.1038/ncomms14905