iTRAQ and virus-induced gene silencing revealed three proteins involved in cold response in bread wheat

• Published in: Scientific reports 2017-08, Vol.7 (1), p.7524-16, Article 7524
• Main Authors: Zhang, Ning, Zhang, Lingran, Zhao, Lei, Ren, Yan, Cui, Dangqun, Chen, Jianhui, Wang, Yongyan, Yu, Pengbo, Chen, Feng
• Format: Article
• Language: English
• Subjects: 13; 14; 38/89; 631/449/2661/2665; 631/45/475; 82; 82/58; Adaptation, Physiological - genetics; Bread - analysis; Carbohydrate metabolism; Cell Wall - chemistry; Cell Wall - metabolism; Cell walls; Cold Temperature; Cold tolerance; Cold-Shock Response; Crosses, Genetic; Electrolyte leakage; Energy metabolism; Gene Expression Regulation, Plant; Gene Silencing; Genes; HSP90 Heat-Shock Proteins - antagonists & inhibitors; HSP90 Heat-Shock Proteins - genetics; HSP90 Heat-Shock Proteins - metabolism; Hsp90 protein; Humanities and Social Sciences; Inbreeding; Lipid metabolism; Metabolic Networks and Pathways - genetics; Metabolism; multidisciplinary; Photosynthesis; Photosynthesis - genetics; Plant Breeding; Plant Cells - chemistry; Plant Cells - metabolism; Plant Proteins - antagonists & inhibitors; Plant Proteins - genetics; Plant Proteins - metabolism; Plant viruses; Plant Viruses - genetics; Plant Viruses - metabolism; Plants, Genetically Modified; Protein turnover; Proteins; Proteomics; Ribonucleic acid; RNA; Science; Science (multidisciplinary); Signal transduction; Sulfur; Transcription; Transduction; Triticum - genetics; Triticum - metabolism; Trypsin Inhibitor, Bowman-Birk Soybean - genetics; Trypsin Inhibitor, Bowman-Birk Soybean - metabolism; Water content; Wheat; Wilting
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-08069-9

By comparing the differentially accumulated proteins from the derivatives (UC 1110 × PI 610750) in the F 10 recombinant inbred line population which differed in cold-tolerance, altogether 223 proteins with significantly altered abundance were identified. The comparison of 10 cold-sensitive descendant lines with 10 cold-tolerant descendant lines identified 140 proteins that showed decreased protein abundance, such as the components of the photosynthesis apparatus and cell-wall metabolism. The identified proteins were classified into the following main groups: protein metabolism, stress/defense, carbohydrate metabolism, lipid metabolism, sulfur metabolism, nitrogen metabolism, RNA metabolism, energy production, cell-wall metabolism, membrane and transportation, and signal transduction. Results of quantitative real-time PCR of 20 differentially accumulated proteins indicated that the transcriptional expression patterns of 10 genes were consistent with their protein expression models. Virus-induced gene silencing of Hsp90, BBI, and REP14 genes indicated that virus-silenced plants subjected to cold stress had more severe drooping and wilting, an increased rate of relative electrolyte leakage, and reduced relative water content compared to viral control plants. Furthermore, ultrastructural changes of virus-silenced plants were destroyed more severely than those of viral control plants. These results indicate that Hsp90, BBI, and REP14 potentially play vital roles in conferring cold tolerance in bread wheat.