Screening, optimization and characterization of exopolysaccharides produced by novel strains isolated from Moroccan raw donkey milk

[Display omitted] •EPS producing bacteria was isolated and identified as Leuconostoc mesenteroides SL and Enterococcus viikkiensis N5.•Optimization was carried out by Response Surface Methodology using Box Behnken Design.•The GC–MS, FTIR, and NMR analysis showed that the EPS-SL and EPS-N5 are hetero...

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Published in:Food Chemistry: X 2022-06, Vol.14, p.100305-100305, Article 100305
Main Authors: Derdak, Reda, Sakoui, Souraya, Pop, Oana Lelia, Cristian Vodnar, Dan, Addoum, Boutaina, Elmakssoudi, Abdelhakim, Errachidi, Faouzi, Suharoschi, Ramona, Soukri, Abdelaziz, El Khalfi, Bouchra
Format: Article
Language:English
Subjects:
antioxidant activity
ass milk
Biological activity
Characterization
Donkey milk
Enterococcus
Exopolysaccharide
exopolysaccharides
food chemistry
leaves
Leuconostoc mesenteroides
molecular weight
Optimization
temperature
thermal stability
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Summary:[Display omitted] •EPS producing bacteria was isolated and identified as Leuconostoc mesenteroides SL and Enterococcus viikkiensis N5.•Optimization was carried out by Response Surface Methodology using Box Behnken Design.•The GC–MS, FTIR, and NMR analysis showed that the EPS-SL and EPS-N5 are heteropolysaccharides connected by α-(1 → 6) and -(1 → 3) linkages.•Both EPSs has high thermal stability.•EPS exhibited appreciable antibacterial and antioxidant activity. Two exopolysaccharides (EPS) producing strains, isolated from raw donkey milk were identified as Leuconostoc mesenteroides SL and Enterococcus viikkiensis N5 using 16S rDNA sequencing. The Box Benheken design exhibited the highest yield of EPS-SL (672.342 mg/L) produced by SL and of EPS-N5 (901 mg/L) produced by N5. The molecular weight was 1.68×104 for EPS-SL and 1.55×104 Da for EPS-N5. FTIR, NMR and GC–MS analysis showed that the EPS are heteropolysaccharides. The SEM image showed that the EPS-SL was smooth and represented a lotus leaf shape and EPS-N5 revealed a stiff-like, porous appearance and was more compact than EPS-SL. The TGA analyses showed high thermal stability and degradation temperature. Additionally, the two EPSs possessed antibacterial and antioxidant activity, and the EPS-SL had the stronger antioxidant activity. Consequently, these results suggest that the functional and biological properties of EPS-SL and EPS-N5 imply the potential application in the food and pharmaceutical industries.
ISSN:2590-1575
2590-1575
DOI:10.1016/j.fochx.2022.100305