Cell vacuolation induced by Haemophilus influenzae supernatants in HEp-2 cells

Haemophilus influenzae belongs to respiratory tract microbiota. We observed vacuoles formation in previous studies with H. influenzae culture supernatants, so in this work we characterised that cytotoxic effect. We observed an abundant production of acidic cytoplasmic vacuoles due to the presence of...

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Bibliographic Details
Published in:Memórias do Instituto Oswaldo Cruz 2013-12, Vol.108 (8), p.1074-1077
Main Authors: Espinoza-Mellado, María del Rosario, López-Villegas, Edgar Oliver, Arteaga-Garibay, Ramón I, Giono-Cerezo, Silvia
Format: Article
Language:English
Subjects:
cell vacuolation
Cells, Cultured
cytotoxicity assays
Cytotoxins - biosynthesis
Cytotoxins - toxicity
Haemophilus influenzae
Haemophilus influenzae - metabolism
Haemophilus influenzae - ultrastructure
Microscopy, Electron, Transmission
PARASITOLOGY
Short Communications
TROPICAL MEDICINE
Vacuoles - microbiology
Vacuoles - ultrastructure
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Summary:Haemophilus influenzae belongs to respiratory tract microbiota. We observed vacuoles formation in previous studies with H. influenzae culture supernatants, so in this work we characterised that cytotoxic effect. We observed an abundant production of acidic cytoplasmic vacuoles due to the presence of a "vacuolating factor" in H. influenzae supernatants which was characterised as thermolabile. Greatest vacuolating activity was observed when utilizing the fraction > 50 kDa. The presence of a large number of vacuoles in HEp-2 cells was verified by transmission electron microscopy and some vacuoles were identified with a double membrane and/or being surrounded by ribosomes. These results suggest similar behaviour to that of vacuolating effects described by autotransporter proteins an undescribed cytotoxic effect induced by H. influenzae.
ISSN:0074-0276
1678-8060
1678-8060
DOI:10.1590/0074-0276130716