A novel and sensitive real-time PCR system for universal detection of poxviruses

Success in smallpox eradication was enabled by the absence of non-human reservoir for smallpox virus. However, other poxviruses with a wider host spectrum can infect humans and represent a potential health threat to humans, highlighted by a progressively increasing number of infections by (re)emergi...

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Bibliographic Details
Published in:Scientific reports 2021-01, Vol.11 (1), p.1798-1798, Article 1798
Main Authors: Luciani, Léa, Inchauste, Lucia, Ferraris, Olivier, Charrel, Rémi, Nougairède, Antoine, Piorkowski, Géraldine, Peyrefitte, Christophe, Bertagnoli, Stéphane, de Lamballerie, Xavier, Priet, Stéphane
Format: Article
Language:English
Subjects:
631/326/2521
631/326/596/1746
Arthropoda
Arthropods
DNA, Viral - genetics
Epidemiology
Genes, Viral
Genomes
Health risks
Humanities and Social Sciences
Humans
Life Sciences
Limit of Detection
multidisciplinary
Phylogeny
Polymerase chain reaction
Poxviridae - genetics
Poxviridae - isolation & purification
Real-Time Polymerase Chain Reaction - methods
Science
Science (multidisciplinary)
Smallpox
Vertebrates
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Summary:Success in smallpox eradication was enabled by the absence of non-human reservoir for smallpox virus. However, other poxviruses with a wider host spectrum can infect humans and represent a potential health threat to humans, highlighted by a progressively increasing number of infections by (re)emerging poxviruses, requiring new improved diagnostic and epidemiological tools. We describe here a real-time PCR assay targeting a highly conserved region of the poxvirus genome, thus allowing a pan-Poxvirus detection (Chordopoxvirinae and Entomopoxvirinae). This system is specific (99.8% for vertebrate samples and 99.7% for arthropods samples), sensitive (100% for vertebrate samples and 86.3% for arthropods samples) and presents low limit of detection (
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-021-81376-4