Self-Assembled Inkjet Printer for Droplet Digital Loop-Mediated Isothermal Amplification

Developing rapid and inexpensive diagnostic tools for molecular detection has been pushed forward by the advancements of technical aspects. However, attention has rarely been paid to the molecular detection methodology using inkjet printing technique. Herein, we developed an approach that employed a...

Full description

Saved in:
Bibliographic Details
Published in:Chemosensors 2022-07, Vol.10 (7), p.247
Main Authors: Fan, Zhaoxuan, Sun, Yucheng, Lin, Jin-Ming
Format: Article
Language:English
Subjects:
digital LAMP
DNA polymerase
Droplets
Gene amplification
Glass substrates
Ink jet printers
inkjet printer
Inkjet printing
Laboratories
Microfluidics
Mineral oils
Printers
Printers (data processing)
Self-assembly
Software
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Developing rapid and inexpensive diagnostic tools for molecular detection has been pushed forward by the advancements of technical aspects. However, attention has rarely been paid to the molecular detection methodology using inkjet printing technique. Herein, we developed an approach that employed a self-assembled inkjet printer as the enabling technology to realize droplet digital loop-mediated isothermal amplification in a low-cost and practical format. An inkjet printer is a self-assembled tool for the generation of discrete droplets in controllable volumes from a picoliter to a nanoliter. A microfluidic chip serves as a droplets reservoir to perform droplet digital LAMP assays. The inkjet printer approach successfully quantified the HPV16 from CaSki cells. This self-assembled and practical inkjet printer device may therefore become a promising tool for rapid molecular detection and can be extended to on-site analysis.
ISSN:2227-9040
2227-9040
DOI:10.3390/chemosensors10070247