Prevention of Clostridium difficile infection in hamsters using a non-toxigenic strain

The present study aimed to evaluate five non-toxigenic strains of Clostridium difficile (NTCD) in vitro and to select one strain to prevent C. difficile (CDI) infection in hamsters ( Mesocricetus auratus ). The NTCD strains were evaluated for spore production in vitro, antimicrobial susceptibility a...

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Bibliographic Details
Published in:Ciência rural 2016-05, Vol.46 (5), p.853-859
Main Authors: Oliveira Júnior, Carlos Augusto de, Silva, Rodrigo Otávio Silveira, Diniz, Amanda Nádia, Pires, Prhiscylla Sadanã, Lobato, Francisco Carlos Faria, Assis, Ronie Antunes de
Format: Article
Language:English
Subjects:
AGRONOMY
Animals
Antimicrobial agents
Antimicrobial resistance
Clindamycin
Clostridium difficile
colite
Competition
diarreia
Disease prevention
doenças associadas à Clostridium difficile
Drug resistance
Esophagus
Genes
Hamsters
Infections
Mesocricetus auratus
Spores
Toxins
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Summary:The present study aimed to evaluate five non-toxigenic strains of Clostridium difficile (NTCD) in vitro and to select one strain to prevent C. difficile (CDI) infection in hamsters ( Mesocricetus auratus ). The NTCD strains were evaluated for spore production in vitro, antimicrobial susceptibility and presence of antimicrobial resistance genes. Approximately 107 spores of the selected strain (Z31) were administered by esophageal gavage in hamsters pretreated with 30mg kg-1 of clindamycin. The challenge with a toxigenic strain of C. difficile was conducted at 36 and 72h, and the animals were observed for 28 days. The NTCD strain of C. difficile (Z31) was able to prevent CDI in all animals that received it. RESUMO: O presente trabalho objetivou a avaliação in vitro de cinco estirpes não toxigênicas de Clostridium difficile (NTCD) e seleção de uma delas para prevenção de infecção por C. difficile (CDI) em hamsters (Mesocricetus auratus ). As estirpes NTCD foram avaliadas quanto à produção de esporos in vitro, sensibilidade antimicrobiana e presença de genes de resistência a antimicrobianos. Aproximadamente 107 esporos da estirpe selecionada (Z31) foram administrados por gavagem esofágica em hamsters previamente tratados com 30mg kg-1 de clindamicina. O desafio com uma estirpe toxigênica de C. difficile ocorreu nos tempos experimentais 36 e 72h, e os animais foram observados por 28 dias. A estirpe NTCD de C. difficile (Z31) foi capaz de prevenir a CDI em todos os animais que a receberam.
ISSN:1678-4596
0103-8478
1678-4596
DOI:10.1590/0103-8478cr20150454