An engineered periosteum for efficient delivery of rhBMP-2 and mesenchymal progenitor cells during bone regeneration

During bone regeneration, the periosteum acts as a carrier for key regenerative cues, delivering osteochondroprogenitor cells and crucial growth factors to the injured bone. We developed a biocompatible, 3D polycaprolactone (PCL) melt electro-written membrane to act as a mimetic periosteum. Poly (et...

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Bibliographic Details
Published in:npj Regenerative medicine 2023-09, Vol.8 (1), p.54-54, Article 54
Main Authors: Romero-Torrecilla, Juan Antonio, Lamo-Espinosa, José María, Ripalda-Cemboráin, Purificación, López-Martínez, Tania, Abizanda, Gloria, Riera-Álvarez, Luis, de Galarreta-Moriones, Sergio Ruiz, López-Barberena, Asier, Rodríguez-Flórez, Naiara, Elizalde, Reyes, Jayawarna, Vineetha, Valdés-Fernández, José, de Anleo, Miguel Echanove-González, Childs, Peter, de Juan-Pardo, Elena, Salmeron-Sanchez, Manuel, Prósper, Felipe, Muiños-López, Emma, Granero-Moltó, Froilán
Format: Article
Language:English
Subjects:
631/61/2035
631/61/490
631/61/54
692/308/575
Biomaterials
Biomedical and Life Sciences
Biomedicine
Bone surgery
Bones
Cell Biology
Collagen
Defects
Fractures
Immunology
Medical research
Orthopedics
Regenerative medicine
Regenerative Medicine/Tissue Engineering
Scanning electron microscopy
Skin & tissue grafts
Stem Cells
Trauma
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Summary:During bone regeneration, the periosteum acts as a carrier for key regenerative cues, delivering osteochondroprogenitor cells and crucial growth factors to the injured bone. We developed a biocompatible, 3D polycaprolactone (PCL) melt electro-written membrane to act as a mimetic periosteum. Poly (ethyl acrylate) coating of the PCL membrane allowed functionalization, mediated by fibronectin and low dose recombinant human BMP-2 (rhBMP-2) (10-25 μg/ml), resulting in efficient, sustained osteoinduction in vitro. In vivo, rhBMP-2 functionalized mimetic periosteum demonstrated regenerative potential in the treatment of rat critical-size femoral defects with highly efficient healing and functional recovery (80%-93%). Mimetic periosteum has also proven to be efficient for cell delivery, as observed through the migration of transplanted periosteum-derived mesenchymal cells to the bone defect and their survival. Ultimately, mimetic periosteum demonstrated its ability to deliver key stem cells and morphogens to an injured site, exposing a therapeutic and translational potential in vivo when combined with unprecedentedly low rhBMP-2 doses.
ISSN:2057-3995
2057-3995
DOI:10.1038/s41536-023-00330-2